奶牛乳腺炎无乳链球菌菌毛岛屿PI-2a骨架蛋白BP基因的克隆及其抗原性预测

发布时间：2018-06-12 04:02

本文选题：无乳链球菌 + 菌毛岛屿　；参考：《中国病原生物学杂志》2017年04期

【摘要】：目的克隆奶牛乳腺炎无乳链球菌菌毛岛屿BP基因片段并进行序列分析。方法根据GenBank已公布牛源无乳链球菌菌毛岛屿PI-2a的BP基因序列,采用Primer 5.0软件设计1对特异性引物,以内蒙古通辽地区分离的乳源性无乳链球菌临床菌株总DNA为模板PCR扩增BP基因片段,并进行生物信息学分析。结果克隆的BP基因序列大小为609bp,编码203个氨基酸残基,其基因序列与GenBank上公布的牛源无乳链球菌菌毛岛屿PI-2a的BP基因(EU930008)相似性为100%。菌毛岛屿骨架蛋白BP二级结构的α螺旋在N端分布较多;β折叠较丰富,且分布于C端较多;无规则卷曲分布较均匀。菌毛岛屿骨架蛋白BP的亲水区分布较广,遍布于整个多肽区,属于亲水性蛋白。BP蛋白分子表面可及性较高,区域占比达57.0%,且抗原性较好。结论奶牛乳腺炎无乳链球菌菌毛骨架蛋白BP基因具有高度保守性,其编码蛋白具有良好的抗原性,可作为防治无乳链球菌性乳腺炎的候选抗原序列。
[Abstract]:Objective to clone and sequence the BP gene of Streptococcus lactis in dairy cow mastitis. Methods A pair of specific primers were designed by using Primer 5.0 software according to the BP gene sequence of PI-2a isolated from Streptococcus lactococcus pili from cattle, which had been published in GenBank. The BP gene fragment was amplified by PCR from the total DNA of Streptococcus lactogenus isolated from Tongliao area of Inner Mongolia and analyzed by bioinformatics. Results the sequence size of the cloned BP gene was 609bp, encoding 203 amino acid residues. The similarity between the BP gene sequence of the cloned BP gene and the BP gene EU930008 of PI-2a, a non-lactobacillus pili island published in GenBank, was 100. The 伪 -helix of BP secondary structure was more in N-terminal, 尾 -fold was abundant and distributed more in C-terminal, and the irregular curl distribution was more uniform. The hydrophilic region of bacterial island skeleton protein BP was widely distributed, and it was found in the whole polypeptide region. It belongs to the hydrophilic protein .BP protein with high surface accessibility, accounting for 57.0% of Prida and having good antigenicity. Conclusion BP gene is highly conserved and its coding protein has good antigenicity. It can be used as a candidate antigen sequence for prevention and treatment of streptococcal mastitis.
【作者单位】：内蒙古民族大学生命科学学院;内蒙古自治区乳源性致病菌防控工程技术研究中心;内蒙古民族大学乳源性致病菌研究所;山东省滨州畜牧兽医研究院;
【基金】：国家自然科学基金项目(No.31560689) 内蒙古自治区乳源性致病菌防控工程技术研究中心开放课题(No.MDK2016036)
【分类号】：S852.61

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