人工诱导方法对白木香萜类相关基因表达量影响研究

发布时间：2018-06-12 06:36

本文选题：白木香 + 人工诱导　；参考：《时珍国医国药》2017年05期

【摘要】：目的研究不同人工诱导方法对白木香结香过程中萜类相关基因表达量的影响。方法 "小孔滴注法"进行甲酸、甲酸结合镰刀菌人工诱导试验,手工割取诱导前、后12个月内的白木香结香部位,实时荧光定量PCR法(qRT-PCR)测定As-HMGR、As-DXS1、As-DXS2基因表达水平。结果 As-HMGR、As-DXS1、As-DXS2回归方程分别为Y=-3.580X+40.11、Y=-3.424X+38.38、Y=-3.728X+41.84,相关系数均大于0.997,线性关系均良好。甲酸、甲酸结合镰刀菌诱导过程中,As-DXS1与As-DXS2的表达模式相近,前8个月表达水平较低,随后显著升高。甲酸诱导时,As-HMGR基因从2个月开始表达,呈现先升高后下降再升高的趋势;甲酸结合镰刀菌诱导时,初期相对表达量缓慢升高,6个月时达到峰值,随后急剧下降。人工诱导12个月As-HMGR、As-DXS1的相对表达量,甲酸诱导高于甲酸结合镰刀菌诱导(P0.05)。结论甲酸、甲酸结合镰刀菌诱导白木香结香过程中As-HMGR基因在伤害早期响应伤害胁迫,As-DXS1、As-DXS2基因在后期响应,为进一步研究白木香萜类次生代谢产物表达与调控,改善人工沉香品质奠定基础。
[Abstract]:Objective to study the effect of different artificial induction methods on the expression of terpene related genes in the process of aroma formation. Methods Formic acid, formic acid combined with Fusarium mongolicum were used for formic acid, formic acid and Fusarium oxysporum to detect the expression level of As-DXS1As-DXS2 gene in As-HMGRN As-DXS1As-DXS2 gene by real-time fluorescence quantitative PCR within 12 months before and 12 months after induction. Results the regression equations of As-HMGRN As-DXS1 and As-DXS2 were Yan-3.580X 40.11X 40.11X 38.324X 38.324X 38.328X 41.84, and the correlation coefficients were all greater than 0.997.The linear relationship was good. The expression patterns of As-DXS1 and As-DXS2 were similar to those of As-DXS2 during the induction of formic acid and formic acid-bound Fusarium. The expression level of As-DXS1 was lower in the first eight months and then increased significantly. The expression of As-HMGR gene began from 2 months after formic acid induction, which increased first and then decreased and then increased. When formic acid was induced by Fusarium oxysporum, the relative expression level increased slowly, reached its peak at 6 months, and then decreased sharply. The relative expression of As-HMGRN As-DXS1 in artificial 12 months was higher than that induced by Fusarium formate. Conclusion in the process of formic acid, formic acid combined with Fusarium, As-HMGR gene responds to injury stress in the early stage of injury. In order to further study the expression and regulation of terpenoid secondary metabolites, As-DXS1 and As-DXS2 genes are in late response to injury stress. Improve the quality of artificial incense to lay the foundation.
【作者单位】：广东药科大学药学院;仙乐健康科技股份有限公司;广东省微生物研究所省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室;
【基金】：国家自然科学基金(No.81102418) 广东省基础与应用基础研究专项资金(No.2014A030313583) 广东省中医药局科研课题(No.20161151) 广东省科学院野外科学实验站基金项目(No.Sytz201504;No.Sytz201511)
【分类号】：S567.19

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