苹果TPS基因家族成员的克隆及其在‘长富2号’花芽孕育中的作用

发布时间：2018-06-12 06:56

本文选题：苹果 + TPS基因家族　；参考：《西北农林科技大学》2017年硕士论文

【摘要】：T6P(海藻糖6-磷酸)是植物碳水化合物状态的指示剂,和蔗糖密切相关,可以作为重要的信号分子参与植物成花诱导。TPS基因家族是T6P生物合成途径的重要成员。本文对苹果TPS基因家族成员进行了鉴定,并对其组织特异性表达进行了分析。同时采用蔗糖喷施处理、摘果处理及品种比较,分析MdTPS基因家族成员在不同碳水化合物水平、不同成花情况下的表达模式,以期探究蔗糖及摘果处理对富士成花的影响,揭示MdTPS基因家族成员对富士花芽孕育的生理与分子机制,结果如下:1.本研究在苹果中鉴定出13个TPS基因家族成员,进化树及基因结构分析表明13个TPS成员被分成两类:MdTPS1、MdTPS2、MdTPS9为Class I亚家族,其余为Class II亚家族;I类TPS基因比II类基因有更多数量的外显子和内含子。此外,在富士苹果中克隆得到了3个I类MdTPS基因,即MdTPS1、MdTPS2、MdTPS9。结果表明MdTPS1、MdTPS2基因序列比基因组中短75bp,MdTPS9基因序列比基因组中长114bp。2.MdTPS基因家族在苹果中的表达具有组织特异性,MdTPS1和MdTPS2具有相似的表达模式,在茎中表达最高,花中表达最低,而MdTPS9在花中表达量最高,在短枝顶芽中表达量最低。II类MdTPS基因中,MdTPS3、4、6、7、11在果实中表达量最高,MdTPS5、12、13在花中表达量最高,MdTPS8、10在芽中表达量最高,说明这些基因可能参与不同的器官建成。3.蔗糖处理和摘果处理促进了富士短枝顶芽可溶性糖含量的积累,加速了成花诱导阶段短枝顶芽的生长,提高了富士成花率。此外,MdTPS家族成员能响应蔗糖处理、摘果处理且在易成花品种‘烟富6号’中表达水平更高。MdTPS1、2、4、11与碳水化合物水平及苹果成花诱导密切相关,可能通过响应蔗糖,调控MdCO、MdSPL2等促进下游花分生组织基因MdLFY、MdAP1来参与苹果成花诱导。4.MdTPS家族成员启动子上含有响应激素(IAA、GA、ABA、JA和SA等)和光照的顺式作用元件,说明MdTPS除了响应蔗糖信号,还可能通过响应激素或光照参与植物成花途径。
[Abstract]:T6P (trehalose 6-phosphoric acid) is an indicator of carbohydrate status in plants and is closely related to sucrose. T6P gene family is an important member of T6P biosynthesis pathway. In this paper, the members of apple TPS gene family were identified and their tissue specific expression was analyzed. At the same time, the expression patterns of members of MdTPS gene family under different carbohydrate levels and flowering conditions were analyzed by sucrose spraying, fruit picking and variety comparison, in order to explore the effect of sucrose and fruit picking on Fuji flower formation. To reveal the physiological and molecular mechanism of MdTPS gene family members on Fuji flower bud development, the results are as follows: 1. In this study, 13 members of TPS gene family were identified in apple. The phylogenetic tree and gene structure analysis showed that 13 TPS members were divided into two categories: MdTPS1, MdTPS2 and MdTPS9 as Class I subfamily. There were more exons and introns in the class I TPS gene than in the class II gene. In addition, three class I MdTPS genes were cloned from Fuji apple, I. E. MdTPS1, MdTPS2 and MdTPS9. The results showed that the MdTPS1 / MdTPS2 gene sequence was 75 bp1 mdTPS9 shorter than that in genome 114bp.2.MdTPS gene family had similar expression patterns in apple, and the expression patterns of MdTPS1 and MdTPS2 were the highest in stem and the lowest in flower. The expression of MdTPS9 was the highest in the flower, and the lowest in the short shoot terminal bud. Class II MdTPS gene was the highest in the fruit. It was suggested that these genes might be involved in the formation of different organs. 3. The highest expression level of MdTPS51212O13 in the flower was the highest in the bud, indicating that these genes might be involved in the formation of different organs. 3. The results showed that the MdTPS9 gene expressed the highest amount in the flower and MdTPS810 showed the highest expression in the flower bud, indicating that these genes might be involved in the formation of different organs. Sucrose treatment and fruit picking treatment promoted the accumulation of soluble sugar in Fuji short shoot terminal bud, accelerated the growth of short branch terminal bud in flower induction stage, and increased Fuji flower formation rate. In addition, MdTPS family members were able to respond to sucrose treatment, fruit picking and higher expression level in 'Yanfu 6', which was closely related to carbohydrate level and apple flower induction, which may be related to sucrose response. Regulation of MdCO-MdSPL2 to promote downstream flower meristem gene MdLFYMdAP1 to participate in flowering induction of apple. 4. The promoter of MdTPS family contains the responsive hormones IAAGA-GAABANJA and SA) and the cis-acting elements of light, which indicate that MdTPS not only responds to sucrose signal, but also to light. It is also possible to participate in the floral pathway of plants by responding to hormones or light.
【学位授予单位】：西北农林科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S661.1

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