牦牛DGAT1基因组织表达及其突变与乳品质性状的关联分析

发布时间：2018-06-12 10:29

本文选题：牦牛 + 二酰基甘油酰基转移酶基因(DGAT)　；参考：《农业生物技术学报》2017年09期

【摘要】：二酰基甘油酰基转移酶1(diacylglycerol acyltransferase 1,DGAT1)作为乳脂性状的重要候选基因,是合成甘油三酯的关键酶。牦牛(Bos grunniens)乳营养丰富,乳脂含量较高;但受分布局域环境、经济及生产条件制约,对其乳品质性状遗传机理研究较少。本研究采用q RT-PCR及单链构象多态性(single strand conformation polymorphism,SSCP)方法,检测牦牛DGAT1基因组织表达,以及3'-UTR和exon8区域多态位点,分析不同基因型及等位基因与甘南牦牛乳品质性状的相关性。结果表明,甘南牦牛DGAT1基因在乳腺、背最长肌、心脏和皮下脂肪高度表达,在肾脏、小肠和大肠中度表达,在脾脏表达最低;4个牦牛群体中DGAT1基因3'-UTR均检测到c.*197 TC的突变,基因型FF个体乳脂率显著高于基因型EE和EF(P0.05),携带等位基因E的个体乳脂率和总固体物质百分含量显著低于未携带者(P0.01或P0.05);甘南牦牛和天祝白牦牛exon 8区检测到c.691-c.692 AAGC(K232A)的双碱基突变,等位基因K为优势等位基因,青海牦牛及野血牦牛该区域内未检测到多态性,推测等位基因K的存在有利于增加牦牛乳脂率。研究结果表明,DGAT1基因3'-UTR区域对牦牛乳质性状有一定的基因效应。本研究可为牦牛乳品质性状分子遗传研究提供基础数据。
[Abstract]:Diacylglycerol acyltransferase (1(diacylglycerol acyltransferase 1) is an important candidate gene for the synthesis of triglycerides. Bos grunniens (Yak) milk was rich in nutrition and high in milk fat content, but the genetic mechanism of milk quality traits was seldom studied due to the local distribution environment, economy and production conditions. In this study, Q RT-PCR and single strand conformation polymorphism (SSCP) were used to detect the expression of DGAT1 gene in yak tissues, and to analyze the correlation between different genotypes and alleles and milk quality traits of Gannan yaks. The results showed that DGAT1 gene was highly expressed in mammary gland, longissimus dorsi muscle, heart and subcutaneous fat, and was moderately expressed in kidney, small intestine and large intestine, and lowest in spleen. The milk fat rate of genotype FF was significantly higher than that of genotype EE and EFP 0.05, and the milk fat rate and percentage of total solid matter in individuals carrying allele E were significantly lower than those of non-carriers P0.01 or P0.05.The c.691-c.692 was detected in exon 8 region of Gannan yak and Tianzhu white yak. The double base mutation of AAGCU K232A, Allele K was the dominant allele, and no polymorphism was detected in Qinghai yak and wild blood yak. It was suggested that the presence of allele K was beneficial to increase the milk fat rate of yak. The results showed that the region of DGAT1 gene 3- UTR had a certain gene effect on milk quality traits of yak. This study can provide basic data for molecular genetic analysis of milk quality traits in yak.
【作者单位】：甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室;甘南州畜牧科学研究所;甘南州动物疫病预防控制中心;
【基金】：甘肃省农业生物技术研究与应用开发项目(No.GNSW-2013-9,No.GNSW-2016-29) 国家自然科学基金项目(No.31160451) 甘肃省杰出青年科学基金项目(No.1210RJDA008)
【分类号】：S823.85

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