日本鳗鲡IFN-γ基因的鉴定、表达模式及启动子活性分析

发布时间：2018-06-12 12:27

本文选题：IFN-γ + 日本鳗鲡　；参考：《水生生物学报》2017年03期

【摘要】：为揭示鱼类IFN-γ的生物学功能,研究从日本鳗鲡(Anguilla japonica)中克隆获得了IFN-γ基因,命名为Aj IFN-γ。Aj IFN-γ具有脊椎动物IFN-γ的典型特征:包括4外显子/3内含子的基因结构、C端的IFN-γ特征性氨基酸基序和1个核定位信号,以及6个α-螺旋反向平行构成的二级结构。Aj IFN-γ在日本鳗鲡所有组织中均低水平转录表达,其中肝脏中表达量最高,其次是皮肤和头肾。Poly I:C刺激和迟缓爱德华氏菌感染均可显著诱导Aj IFN-γ在鳃、头肾、体肾和(或)脾脏中的转录表达,表明Aj IFN-γ能够参与日本鳗鲡抗菌和抗病毒的免疫过程。此外,研究还克隆了Aj IFN-γ基因的5′调控区序列共1536 bp,并构建了一系列Aj IFN-γ5′调控区删节突变体,分析其启动子活性,结果表明,上游 240/+136区域中含有起始Aj IFN-γ转录的关键启动子调控元件, 1062/ 814区域存在转录的正调控元件,而 1252/ 1062区域存在转录的负调控元件。上述结果进一步丰富了鱼类IFN-γ的基础知识。
[Abstract]:In order to reveal the biological function of fish IFN- 纬, IFN- 纬 gene was cloned from Anguilla japonica of Japanese eel. Named AjIFN- 纬. AjIFN- 纬 has typical characteristics of vertebrate IFN- 纬: IFN- 纬 characteristic amino acid motif of C-terminal and a nuclear localization signal, including the gene structure of exon 4 / 3 intron. The expression of AjIFN- 纬 in all tissues of Japanese eel was lower than that in the liver, and the secondary structure composed of 6 伪 -helix reverse parallelism was the highest, and the expression of AjIFN- 纬 was the highest in the liver of Japanese eel. Secondly, the transcription expression of AjIFN- 纬 in Gill, head kidney, body kidney and / or spleen was significantly induced by skin and head-kidney. Poly I: C stimulation and Edouard tardy infection, indicating that Aj IFN- 纬 can participate in the anti-bacterial and anti-virus immune process of Japanese eel (Anguilla japonica). In addition, the 5'regulatory region of AjIFN- 纬 gene was cloned into 1536 BP, and a series of abridged mutants of AjIFN- 纬 5 'regulatory region were constructed, and the promoter activity was analyzed. There are key promoter regulatory elements for initiation of AjIFN- 纬 transcription in the upstream region of 240 / 136, positive regulatory elements for transcription in the 1062 / 1062 region, and negative regulatory elements for transcription in the 1252 / 1062 region. These results further enrich the basic knowledge of fish IFN- 纬.
【作者单位】：集美大学水产学院;中国科学院水生生物研究所淡水生态与生物技术国家重点实验室;鳗鲡现代产业技术教育部工程研究中心;福建省海洋生物资源开发利用协同创新中心;
【基金】：国家自然科学基金(31402329;31174238和U1205123) 福建省自然科学基金(2014J05042;2012J06008和JK2014026) 鳗鲡工程中心科研基金资助~~
【分类号】：S917.4

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