甘蓝型油菜BnMYB80基因的生物信息学分析

发布时间：2018-06-12 13:06

  本文选题：生物信息学 + 甘蓝型油菜　； 参考：《植物学报》2016年05期

【摘要】：在高等植物花药发育和花粉形成中,MYB转录因子起着非常重要的作用,其中MYB80是参与绒毡层发育及引起雄性不育的重要转录因子。该研究以拟南芥(Arabidopsis thaliana)At MYB80为参考序列,通过BLAST比对分析,在白菜(Brassica rapa)、甘蓝(B.oleracea)和甘蓝型油菜(B.napus)中分别获得MYB80基因的2、2和6个同源序列,运用生物信息学方法对其核苷酸序列及编码的氨基酸序列进行组成成分、亚细胞定位、磷酸化位点、疏水性/亲水性、蛋白质二级、三级结构和功能域分析。结果表明,MYB80转录因子亚细胞定位于细胞核,具有多个不同的磷酸化位点,肽链表现为亲水性;二级、三级结构预测显示,MYB80蛋白以α-螺旋和无规则卷曲为主要结构元件;保守结构域分析表明,其N端具有2个串联的SANT功能域,属于R2R3型MYB转录因子。多重序列比对和进化树分析结果表明,甘蓝型油菜与白菜、甘蓝的序列相似性大于92%,且MYB80转录因子的功能结构域具有较高的同源性和较强的序列保守性。该研究结果对深入解析甘蓝型油菜MYB80的生物学功能及育性调控的分子机理具有重要意义,为甘蓝型油菜杂种优势利用提供了依据。
[Abstract]:MYB transcription factors play a very important role in anther development and pollen formation of higher plants, among which MYB80 is an important transcription factor involved in tapetum development and male sterility. Using Arabidopsis thaliana)At MYB80 as the reference sequence and blast comparison analysis, two and six homologous sequences of MYB80 gene were obtained in Brassica rapaena, B. oleracea and Brassica napus, respectively. The composition, subcellular localization, phosphorylation site, hydrophobic / hydrophilic, protein secondary, tertiary structure and functional domain were analyzed by bioinformatics. The results showed that the MYB80 transcription factor subcells were located in the nucleus and had many different phosphorylation sites, and the peptide chain was hydrophilic, the second and third order structure prediction showed that the main structural elements of MYB80 protein were 伪 -helix and irregular curl. The analysis of conserved domain showed that the N-terminal had two sant domains, which belonged to R2R3 MYB transcription factor. The results of multiple sequence alignment and phylogenetic tree analysis showed that the sequence similarity between Brassica napus and Brassica campestris was higher than that in Brassica napus, and the functional domain of MYB80 transcription factor had high homology and strong conserved sequence. The results of this study are of great significance in understanding the biological function of MYB80 and the molecular mechanism of fertility regulation in Brassica napus, and provide a basis for the utilization of heterosis in Brassica napus L.
【作者单位】： 西南大学农学与生物科技学院;
【基金】：国家重点研发计划“七大农作物育种”专项(No.2016YFD0101300) 国家自然科学基金(No.U1302266)
【分类号】：S565.4
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本文编号：2009772