草鱼不同部位肌肉营养成分、肌纤维特性以及脂肪代谢相关基因的表达

发布时间：2018-06-12 13:05

本文选题：草鱼(Ctenopharyngodon + idella)　；参考：《淡水渔业》2017年02期

【摘要】：为了探讨草鱼(Ctenopharyngodon idella)不同部位肌肉品质的差异,本研究选取体质量为(1 026.10±145.55)g的草鱼,检测了背部、腹上部、腹底部和尾部肌肉的营养成分、肌纤维组织结构特性以及脂肪代谢相关基因的表达。结果表明,草鱼腹底部肌肉的粗脂肪含量显著高于背部、腹上部和尾部肌肉,而水分含量则显著降低;背部肌肉粗蛋白含量显著高于腹上部、腹底部和尾部肌肉。腹上部肌肉的肌纤维直径显著高于背部、腹底部和尾部肌肉,但其肌纤维密度则显著降低,而最高的肌纤维密度出现在腹底部肌肉;草鱼背部和尾部肌肉肌纤维密度无显著差异。草鱼腹底部肌肉的脂蛋白脂肪酶基因mRNA表达量显著高于草鱼背部、腹上部和尾部肌肉。脂肪酸合成酶和乙酰辅酶A羧化酶α的mRNA表达量在草鱼背部、腹上部、腹底部和尾部肌肉之间无显著差异。研究表明,草鱼不同部位的肌肉营养特性存在显著性差异,肌肉脂肪含量与肌纤维直径呈负相关,与脂蛋白脂肪酶基因的表达量呈正相关。
[Abstract]:In order to investigate the difference of muscle quality in different parts of Ctenopharyngodon idella, grass carp with a body weight of 1 026.10 卤145.55 g was used to detect the nutritional components of the back, upper abdomen, ventral bottom and tail muscles. Structural characteristics of muscle fibers and expression of genes related to fat metabolism. The results showed that the content of crude fat in the muscle of the bottom of the abdomen was significantly higher than that in the back, the muscle in the upper part of the abdomen and the tail muscle, while the content of water in the muscle of the back muscle was significantly lower than that in the upper part of the abdomen, the bottom of the abdomen and the tail muscle. The muscle fiber diameter of upper abdominal muscle was significantly higher than that of back, ventral bottom and tail muscles, but the density of muscle fiber decreased significantly, while the highest density of muscle fiber appeared in the muscle of abdominal base. There was no significant difference in muscle fiber density between the back and tail muscles of grass carp. The mRNA expression of lipoprotein lipase gene in the muscle of the bottom of grass carp was significantly higher than that in the back, upper abdomen and tail muscles of grass carp. There was no significant difference in the mRNA expression of fatty acid synthase and acetyl coenzyme A carboxylase 伪 between the dorsal, upper abdomen, bottom and tail muscles of grass carp. The results showed that there were significant differences in nutritional characteristics between different parts of grass carp muscle. Muscle fat content was negatively correlated with muscle fiber diameter and positively correlated with the expression of lipoprotein lipase gene.
【作者单位】：华中农业大学水产学院池塘健康养殖湖北省工程实验室;
【基金】：现代农业产业技术体系建设专项资金(CARS-46) 国家自然科学基金(31502140) 中央高校基本科研业务费专项资金资助项目(2662016QD047,2662015PY119)
【分类号】：S917.4

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