河北3个日本沼虾野生群体线粒体DNA D-Loop基因序列变异及种群遗传结构分析

发布时间：2018-06-13 01:07

本文选题：日本沼虾 + D-Loop基因　；参考：《水产学报》2017年02期

【摘要】：为进一步了解白洋淀与衡水湖日本沼虾种质资源现状,实验通过对白洋淀南淀(SB)、白洋淀北淀(NB)、衡水湖(HS)日本沼虾群体线粒体DNA D-Loop基因片段进行扩增和测定,得到935 bp的序列用于进一步的分析,其中变异位点80个,占分析位点的8.56%,含有77个简约信息位点,平均转颠换比值(TS/TV)为7.28,4种碱基在所得序列中平均含量为A(42.65%)、G(9.08%)、T(36.98%)、C(11.29%),其中A+T含量为79.63%,明显高于C+G含量。AMOVA分析结果显示,群体间的遗传分化系数FST=0.2336,群体间遗传变异占23.36%,群体内遗传变异占76.64%,群体间具有较高程度的遗传分化。运用K-2-P模型构建了3群体的23个单倍型的NJ系统发育树,单倍型之间并没有按照单倍型所属群体的分类聚簇,表现为不同群体个体相互交错形成复杂的簇群。群体中性检验、错配分析表明,日本沼虾近期未曾经历过种群扩张。
[Abstract]:In order to further understand the current situation of germplasm resources of Macrobrachium japonicus in Baiyangdian and Hengshui Lake, the D-Loop gene fragment of mitochondrial DNA of Japanese prawn populations was amplified and determined by means of amplification and determination of SBP, NBX and HSH in Baiyangdian, Baiyangdian and Hengshui Lake, respectively. A 935 BP sequence was obtained for further analysis. Among them, there were 80 mutation sites, accounting for 8.56% of the analysis sites, and there were 77 parsimonious information sites. The average transposition ratio (T / T) was 7.28. The average content of the four bases in the sequence was 42.65%, and the average content was 36.98%, and the A T content was 79.63 3, which was significantly higher than that of C G content. AMOVA analysis showed that The coefficient of genetic differentiation between populations is 0.2336, the genetic variation among populations is 23.36, the genetic variation within populations is 76.64, and there is a high degree of genetic differentiation among populations. Using K-2-P model, 23 NJ phylogenetic trees of 3 populations were constructed. The haplotypes did not cluster according to the classification of haplotypes, which showed that the individuals of different populations interlaced with each other to form complex clusters. Population neutral test and mismatch analysis showed that Macrobrachium japonicus had not experienced population expansion recently.
【作者单位】：河北大学生命科学学院;河北省海洋与水产科学研究院;衡水市水产技术推广站;
【基金】：河北省现代农业产业技术体系淡水养殖创新团队项目~~
【分类号】：S917.4

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