苹果生长素运输基因MdABCB19的克隆及其在矮化砧木中的表达分析

发布时间：2018-06-13 01:12

本文选题：苹果 + 生长素　；参考：《园艺学报》2017年03期

【摘要】：以苹果矮化砧木M9和乔化砧木MM106为材料,克隆到一个3 753 bp的核苷酸序列,其编码1 250个氨基酸,含有两个ABC_membrance结构域,两个ABC_tran结构域,与梨、油菜和拟南芥的ABCB19基因高度同源,命名为MdABCB19。该序列在M9和MM106中存在一个非同义SNP,编码氨基酸由A突变为S,导致M9α螺旋少了一段。表达量分析表明,MdABCB19在砧木M9和MM106、长富2号/M9和长富2号/MM106均差异表达。启动子序列分析发现M9的MdABCB19启动子在起始密码子上游170 bp处有6个碱基(CTCTGT)缺失,导致缺失一个5'UTR Py-rich stretch motif。MM106的MdABCB19启动子活性高于M9,并且受光照调控。推测M9的MdABCB19启动子5'UTR Py-rich stretch motif缺失可能与其MdABCB19低表达有关;而氨基酸突变导致蛋白质三级结构α螺旋结构改变是否影响生长素运输,有待进一步研究。上述研究表明苹果MdABCB19可能通过调控生长素转运参与砧木苗矮化性状的调控。
[Abstract]:A 3 753 BP nucleotide sequence was cloned from apple dwarf rootstock M9 and arborization rootstock MM106. It encodes 1 250 amino acids and contains two ABCmembrance domains and two ABCtran domains, which are highly homologous to the ABCB19 gene of pear, rape and Arabidopsis thaliana. Named MdABCB19. There is a nonsynonymous SNPs in M9 and MM106. The encoding amino acids are mutated from A to S, resulting in a loss of M9 伪 helix. The expression of MdABCB19 in stock M9 and MM106, Changfu 2 / M9 and Changfu 2 / MM106 were analyzed. The promoter sequence analysis showed that MdABCB19 promoter of M9 had six base CTGTs deletion at 170bp upstream of the start codon, which resulted in the deletion of a 5UUTR Py-rich stretch motif.MM106 promoter activity higher than that of M9 promoter, and was regulated by light. It is speculated that the deletion of MdABCB19 promoter 5UTR Py-rich stretch motif may be related to the low expression of MdABCB19, and whether amino acid mutation leads to the change of protein tertiary structure 伪 helix structure may affect auxin transport. These results suggest that MdABCB19 may be involved in the regulation of dwarfing characters of rootstocks by regulating auxin transport.
【作者单位】：西北农林科技大学园艺学院国家苹果改良中心杨凌分中心陕西果业发展协同创新中心;
【基金】：国家现代农业产业技术体系建设专项资金项目(CARS-28) 国家星火计划项目(2014GA850002) 陕西科技创新工程项目(2015NY114) 陕西省科技统筹创新工程计划项目(2016KTZDNY01-10)
【分类号】：S661.1

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