刺五加MDD基因启动子的克隆与生物信息学分析

发布时间：2018-06-13 02:29

  本文选题：刺五加 + MDD基因　； 参考：《中草药》2016年21期

【摘要】：目的克隆并分析刺五加甲羟戊酸焦磷酸脱羧酶(mevalonate diphosphate decarboxylase,MDD)基因的启动子序列。方法根据刺五加MDD基因的c DNA序列,采用PCR扩增和热不对称交错PCR(TAIL-PCR)技术,克隆MDD基因5’端的DNA序列及启动子序列。利用Plant CARE等软件对其进行生物信息学分析。结果克隆得到长1 423 bp的刺五加MDD基因启动子序列及长1 024 bp的5’端DNA序列。该启动子序列含有49个TATA-box、25个CAAT-box。还含有脱落酸响应元件、茉莉酸甲酯响应元件、胚乳表达必须顺式作用元件、干旱胁迫响应元件、光响应元件等多种顺式作用元件,以及2个MYBHv1与2个MBY结合位点。结论首次克隆并分析了刺五加MDD基因的启动子序列,为该基因的表达调控奠定了基础。
[Abstract]:Objective to clone and analyze the promoter sequence of mevalonate diphosphate decarboxylase (MDD) gene from Acanthopanax senticosus (Acanthopanax senticosus). Methods according to the cDNA sequence of MDD gene of Acanthopanax senticosus, the 5 '-terminal DNA sequence and promoter sequence of MDD gene were cloned by PCR amplification and thermal asymmetric interleaving PCR. The bioinformatics analysis was carried out by means of Plant care and other software. Results the promoter sequence of MDD gene of Acanthopanax senticosus and the 5 'terminal DNA sequence of 1 024 BP were cloned. The promoter contains 49 TATA-box and 25 CAAT-box. It also contains abscisic acid response element, methyl jasmonate response element, endosperm expression must be cis-acting element, drought stress response element, photoresponse element, and two MYBHv1 binding sites to two MBY. Conclusion the promoter sequence of MDD gene of Acanthopanax senticosus was cloned and analyzed for the first time.
【作者单位】： 华北理工大学生命科学学院;
【基金】：国家自然科学基金项目(31570683) 河北省教育厅资助科研项目(QN2014102) 华北理工大学培育基金(SP201508);华北理工大学大学生创新创业训练计划(X2015170)
【分类号】：S567.19
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本文编号：2012256