白叶枯病菌侵染下的水稻内参基因稳定性

发布时间：2018-06-13 05:20

本文选题：水稻白叶枯 + 水稻内参基因　；参考：《分子植物育种》2017年01期

【摘要】：水稻白叶枯病是由黄单胞菌水稻致病变种引起的一种细菌性枯萎病害,严重危害水稻产量。水稻受白叶枯侵染后,通过对相关基因表达变化进行检测,是研究水稻-白叶枯病菌分子互作机理的一种重要手段。为了筛选出稳定表达的内参基因,以确保后续基因表达分析结果的可靠性,本研究以接种了白叶枯病菌PXO61、PXO99的水稻叶片为研究材料,在侵染后不同时间点取样,对6个常用的水稻内参基因(TUB,EF1α,UBQ5,ACT,18Sr RNA和GAPDH)的表达进行qRT-PCR检测并用ge Norm、Norm Finder和Best Keeper三种软件对6对常用内参基因的稳定性进行了分析。结果发现:6个内参基因在两种白叶枯病菌侵染后的表达量变化均有差异;综合三种软件算法,受白叶枯PXO61和PXO99侵染后水稻叶片中表达最稳定的基因为EF1α、ACT和TUB,为水稻响应白叶枯病菌基因表达及其进一步的功能研究提供了参考和借鉴。
[Abstract]:Rice bacterial blight is a bacterial wilt caused by xanthomonas oryzae. It is an important means to study the molecular interaction mechanism of rice and bacterial blight by detecting the changes of gene expression in rice infected with white leaf blight. In order to screen the stably expressed internal reference genes and ensure the reliability of the results of subsequent gene expression analysis, the leaves of rice inoculated with bacterial blight fungus PXO61 and PXO99 were used as the research materials, and the samples were taken at different time points after infection. The expression of 18 Sr RNA and GAPDH of six common rice internal reference genes, TUBQ5, EF1 伪, and GAPDH, were detected by qRT-PCR, and the stability of 6 common reference genes were analyzed by ge Norm Finder and Best Keeper. The results showed that there were differences in the expression levels of the six internal reference genes after infection by two bacterial blight bacteria. The most stable genes expressed in rice leaves infected by PXO61 and PXO99 were EF1 伪 ACT and TUB, which provided reference and reference for the gene expression and further functional study of rice bacterial blight responsive bacteria (PXO61 and PXO99).
【作者单位】：海南大学农学院;海南省热带生物资源可持续利用重点实验室;
【基金】：海南省科学自然基金(20153071) 海南大学科研启动基金(Kyqd1518)共同资助
【分类号】：S435.111.47

【相似文献】