内蒙古地区乳源金黄色葡萄球菌肠毒素基因的分布

发布时间：2018-06-13 10:47

本文选题：内蒙古地区 + 金黄色葡萄球菌　；参考：《黑龙江畜牧兽医》2017年17期

【摘要】：为了了解内蒙古地区金黄色葡萄球菌肠毒素基因的分布状况,并为评估金黄色葡萄球菌肠毒素的污染风险和研制金黄色葡萄球菌疫苗提供基础资料,试验采用PCR方法,对2015年从内蒙古地区临床型奶牛乳房炎乳样中分离的121株金黄色葡萄球菌进行16种肠毒素基因的检测。结果表明:121株金黄色葡萄球菌全部携带肠毒素基因,其中携带比例较高的是sei、selm和selj基因,分别为121株(100%)、81株(66.94%)和64株(52.89%),未检测到see、selo和selp基因。携带传统肠毒素基因(sea-see)的有59株(48.76%),携带新型肠毒素基因(seg-selq)的有121株(100%)。同时携带2种及2种以上肠毒素毒基因的有118株(97.52%),组合也很复杂。说明内蒙古地区的奶牛场存在着很大的金黄色葡萄球菌安全隐患,防治较为困难。
[Abstract]:In order to understand the distribution of Staphylococcus aureus enterotoxin gene in Inner Mongolia, and to provide basic information for evaluating the risk of Staphylococcus aureus enterotoxin and the development of Staphylococcus aureus vaccine, the PCR method was used to detect 121 strains of golden yellow from the milk samples of dairy cow mastitis in Inner Mongolia area in 2015. 16 enterotoxin genes were detected in Staphylococcus aureus. The results showed that 121 strains of Staphylococcus aureus carrying enterotoxin genes were all carrying SEI, Selm and SELJ genes, 121 (100%), 81 (66.94%) and 64 (52.89%), and no see, selo and SELP genes were detected. There were 59 with the traditional enterotoxin gene (sea-see). Plant (48.76%), 121 strains (100%) carrying new enterotoxin gene (seg-selq). 118 strains of 2 or more than 2 enterotoxigenic genes (97.52%) were carried at the same time, and the combination was very complex. It showed that the dairy farm in Inner Mongolia area had a large safety hidden danger of Staphylococcus aureus and it was more difficult to prevent and cure.
【作者单位】：内蒙古农业大学兽医学院;
【基金】：“十二五”国家科技支撑计划项目“重点牧区‘生产生态生活’保障技术集成与示范”(2012BAD13BOO)
【分类号】：S852.611

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