不同海拔猪血液抗氧化指标与耳组织相关基因表达的差异分析

发布时间：2018-06-13 08:26

本文选题：抗氧化指标 + 血液　；参考：《黑龙江畜牧兽医》2017年09期

【摘要】：为了研究不同海拔对猪血液中抗氧化指标和耳组织中相关基因表达是否存在差异,试验选择具有明显海拔高度差异的迪庆藏猪(3 500 m)和保山猪(2 600 m)作为研究对象,分别采集5月龄迪庆藏猪、保山猪的血液和耳组织,检测血清中一氧化氮(NO)、丙二醛(MDA)含量以及总超氧化物歧化酶(T-SOD)、过氧化物酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的活力,并检测耳组织中SOD、CAT和GSH-Px基因表达的变化。结果表明:两个品种猪血清中NO、MDA含量和GSH-Px的活力没有显著性差异。迪庆藏猪血清中CAT的活力显著高于保山猪(P0.05);T-SOD的活力极显著高于保山猪(P0.01);此外,迪庆藏猪耳组织中CAT的基因表达显著高于保山猪(P0.05);SOD的基因表达水平极显著高于保山猪(P0.01);GSH-Px的基因表达水平有显著高于保山猪的趋势(P=0.071)。说明一定的海拔高度能够使猪产生氧化应激,高海拔的猪抗氧化应激能力更强。
[Abstract]:In order to study the difference of antioxidant index in blood and the expression of related genes in ear tissue of pigs at different elevations, we selected Diqing Tibetan pig (3 500 m) and Baoshan pig (2 600 m) as the research objects. The blood and ear tissues of 5-month-old Diqing Tibetan pigs and Baoshan pigs were collected respectively. The contents of nitric oxide (no) and malondialdehyde (MDA) in serum and the activities of total superoxide dismutase (T-SOD), peroxidase (CAT) and glutathione peroxidase (GSH-Px) were measured. The changes of cat and GSH-Px gene expression in ear tissue were detected. The results showed that there was no significant difference in the content of MDA and the activity of GSH-Px in serum between the two breeds. The activity of cat in serum of Diqing Tibetan pig was significantly higher than that of P0.05 and T-SOD in Baoshan pig, and the activity of T-SOD was significantly higher than that of P0.01in Baoshan pig. The expression level of cat gene was significantly higher in Diqing Tibetan pig ear than that in Baoshan pig P0.05 and SOD. The expression level of cat gene was significantly higher than that of Baoshan pig P0.01 and GSH-Px. The level of cat gene expression was significantly higher than that of Baoshan pig P0.071. The results showed that high altitude could induce oxidative stress in pigs, and the antioxidant stress ability of high altitude pigs was stronger than that of high altitude pigs.
【作者单位】：大理大学农学与生物科学学院;
【基金】：云南省教育厅项目(2014C110Y) 大理大学博士科研启动基金项目(KYBS201406)
【分类号】：S828

【相似文献】