弱精子症患者精子外周致密纤维2基因及蛋白的表达

发布时间：2018-06-14 18:38

本文选题：外周致密纤维 + 弱精子症　；参考：《中华男科学杂志》2017年11期

【摘要】：目的:探讨弱精子症患者精子中外周致密纤维2(ODF2)mRNA及蛋白的表达水平,比较ODF2在健康男性与弱精子症患者中的表达差异。方法:根据WHO第5版,收集身体健康无疾病正常男性精液(n=15)和弱精子症患者精液(n=45)共60份,利用计算机辅助精液分析系统(CASA)进行精子统计,将弱精子症分为轻、中、重度3组,采用实时定量PCR和Western印迹对4组标本进行ODF2基因的表达量研究。结果:与正常组相比,轻度弱精子症组精子中ODF2基因表达无明显统计学差异(1.1120±0.5255 vs 0.6880±0.3720,P0.05);中度弱精子症组ODF2基因表达降低,有显著的统计学差异(1.1120±0.5255 vs 0.4833±0.1863,P0.05);重度弱精子症组ODF2基因表达明显降低,有极显著的统计学差异(1.1120±0.5255 vs 0.448 3±0.3408,P0.01)。Western印迹中ODF2的OD值(ODF2/β-actin)在正常组和轻、中、重度弱精子症组分别为:0.4587±0.0521、0.3261±0.0714、0.1454±0.0536和0.1227±0.0457,与正常组相比,轻度弱精子症组无明显统计学差异(P0.05),中度弱精子症组有显著的统计学差异(P0.05),重度弱精子症组有极显著的统计学差异(P0.01)。结论:ODF2基因及蛋白在弱精子症患者精子中的表达下调与精子活动力下降有密切联系,呈正相关性,提示ODF2基因可能参与精子活力的调控。
[Abstract]:Objective: to investigate the expression level of ODF 2 mRNA and protein in spermatozoa of asthenospermia patients, and to compare the difference of expression of ODF2 between healthy men and asthenospermia patients. Methods: according to the 5th edition of WHO, 60 semen samples were collected from healthy and disease-free normal men and semen from patients with asthenospermia. The sperm count was carried out by CASA-CASA.All the patients were divided into three groups: mild, moderate and severe. The expression of ODF2 gene in 4 groups was studied by real time quantitative PCR and Western blotting. Results: there was no significant difference in the expression of ODF2 gene between the mild asthenospermia group and the normal group (1.1120 卤0.5255 vs 0.6880 卤0.3720 P0.05A), while the expression of ODF2 gene in the moderate asthenospermia group was lower than that in the moderate asthenospermia group. The expression of ODF2 gene in severe asthenospermia group was significantly lower than that in severe asthenospermia group (1.1120 卤0.5255 vs 0.448 3 卤0.3408P0.01U. Western blotting), and the OD value of ODF2 / 尾 -actinin was significantly lower in the normal group than in the mild group (P 0.05), while the expression of ODF2 gene in the severe asthenospermia group was significantly lower than that in the control group (P < 0.05), while the expression of ODF2 gene in the severe asthenospermia group was significantly lower than that in the control group. The proportion of severe asthenospermia was 0.4587 卤0.0521 卤0.3261 卤0.07140.1454 卤0.0536 and 0.1227 卤0.0457 respectively. There was no significant difference between mild asthenospermia group and normal group (P 0.05), but there was significant difference in moderate asthenospermia group (P 0.05) and severe asthenospermia group (P 0.01). Conclusion the down-regulation of the expression of the 1: ODF2 gene and protein in the spermatozoa of asthenospermia patients is closely related to the decrease of sperm motility, which suggests that the ODF2 gene may be involved in the regulation of sperm motility.
【作者单位】：成都中医药大学第二附属医院生殖医学中心;成都中医药大学第二附属医院检验科;
【基金】：四川省科技厅科研院所成果转化项目(15010116) 成都市科技项目(2014-HM01-00187-SF) 成都中医药大学校基金(ZRQN1402);成都中医药大学科技发展基金(ZRYY1635)~~
【分类号】：R698.2

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