鸡MOGAT和DGAT基因的克隆及表达特性研究

发布时间：2018-06-15 11:10

  本文选题：鸡 + 脂类代谢　； 参考：《畜牧兽医学报》2017年02期

【摘要】：本研究旨在探讨鸡MOGAT和DGAT基因的表达和调控特性。首先克隆了具有MOGAT活性的MOGAT1、MOGAT2、LPGAT1和具有DGAT活性的DGAT2和SOAT1对应基因的编码区序列,并对其组织表达特性进行了研究;其次选取产蛋前期母鸡和产蛋高峰期母鸡作为试验材料,利用实时荧光定量PCR技术分析不同生理时期鸡肝中这些基因的表达变化规律;在此基础上利用雌激素处理的体内、体外试验,分析这些基因的表达调控机制。结果表明,鸡MOGAT1、MOGAT2、LPGAT1、DGAT2和SOAT1基因在各组织中表现出广泛表达的特性,并在脂类代谢旺盛的器官如肝、肾、小肠(十二指肠、空肠、回肠)等器官中表达较高,但DGAT2基因在鸡肝中的表达量较低,而MOGAT2基因主要在鸡小肠组织(十二指肠、空肠、回肠)中表达,MOGAT1在除胰腺外的大部分组织中都有表达;产蛋高峰期母鸡肝中MOGAT1、LPGAT1和SOAT1的表达量均显著低于产蛋前期母鸡;经不同浓度雌激素处理后,这些基因在鸡肝、十二指肠、肾以及鸡胚肝原代细胞中的表达量维持不变或显著下调。综上所述,已知的甘油三酯合成的单酰甘油通路中相关基因不是鸡肝TG合成代谢中的限速基因,在雌激素诱导的鸡TG合成代谢中,单酰甘油通路不发挥主导作用。
[Abstract]:The purpose of this study was to investigate the expression and regulatory characteristics of MOGAT and DGAT genes in chickens. The coding region sequences of MOGAT active MOGAT1 / MOGAT2 LPGAT1 and DGAT active DGAT2 and SOAT1 corresponding genes were cloned, and their tissue expression characteristics were studied. Secondly, early laying hens and laying peak hens were selected as experimental materials. The expression of these genes in chicken liver at different physiological stages was analyzed by real-time fluorescence quantitative PCR, and the regulation mechanism of these genes was analyzed by estrogen treatment in vivo and in vitro. The results showed that the genes of MOGAT1, MOGAT2, LPGAT1, DGAT2 and SOAT1 were widely expressed in various tissues, and were highly expressed in organs with high lipid metabolism, such as liver, kidney, small intestine (duodenum, jejunum, ileum) and so on. However, the expression of DGAT2 gene in chicken liver was low, while MOGAT2 gene was mainly expressed in small intestine (duodenum, jejunum, ileum) of chicken. The expression of MOGAT1 LPGAT1 and SOAT1 in the liver of hens during peak laying period was significantly lower than that in prelaying hens, and these genes were treated with different concentrations of estrogen in the liver and duodenum of chickens. The expression of renal and chicken embryonic liver primary cells remained unchanged or significantly down-regulated. In conclusion, the known genes related to triglyceride synthesis are not rate-limiting genes in TG synthesis metabolism of chicken liver, but not in estrogen-induced TG synthesis metabolism.
【作者单位】： 河南农业大学牧医工程学院河南省家禽种质资源创新工程研究中心河南省家禽育种国际联合实验室;
【基金】：河南省国际合作项目(162102410030) 国家自然科学基金(31372330) 农业部现代农业产业技术体系(CARS-41-K04) 河南省重大科技攻关计划项目(112101110800)
【分类号】：S831.2
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本文编号：2021806