HMA基因家族在芸薹属AC基因组中的进化分析

发布时间：2018-06-15 13:06

本文选题：芸薹属 + AC基因组　；参考：《中国油料作物学报》2017年03期

【摘要】：为研究芸薹属AC基因组重金属转运ATP酶(HMA,heavy metal transporting ATPase)参与的金属转运机制,利用白菜、甘蓝和甘蓝型油菜全基因组数据对HMA基因进行全基因组水平鉴定,并分析其在芸薹属AC基因组中的进化。结果显示,在芸薹属AC基因组中共鉴定到50个HMA基因,其中白菜14个、甘蓝13个、甘蓝型油菜23个(A亚基因组11个,C亚基因组12个),并且HMA基因在三个物种中均存在不均匀加倍的现象。这50个基因分属于P_1B-1、P_1B-2、P_1B-4三个亚族,在蛋白保守序列分布、蛋白理化性质、基因结构和启动区顺式作用元件等方面不同亚族之间各有差异,但同一亚族的基因在组织表达特异性方面也存在较大的差异。还发现Bra003110、Bol030251、BnaA10g06240D、BnaC09g28870D,BnaC09g11230D、BnaC04g20290D、BnaA09g10950D和Bra027641几个与At HMA5同源的基因,在多个方面较At HMA5发生了较大的变异,可能会导致这些基因在芸薹属作物中延伸出不同于At HMA5的功能。在芸薹属AC基因组HMA基因中发现的GICCSME和GICCPSE新变异类型,不同于拟南芥中的GICC(T/S)SE功能位点,也可能导致相应的基因出现新的功能变异。本研究为从芸薹属AC基因组中选择特异性金属离子转运的HMA基因提供生物信息学参考,对进一步解析芸薹属植物中HMA基因参与的金属离子转运机制具有重要的指导意义。
[Abstract]:In order to study the mechanism of heavy metal transport in Brassica (Brassica) AC genome, the whole genome data of Brassica chinensis, Brassica napus and Brassica napus were used to identify the whole genome level of HMA gene. Its evolution in the AC genome of Brassica was analyzed. The results showed that 50 HMA genes were identified in the AC genome of Brassica, including 14 in Chinese cabbage and 13 in cabbage. In Brassica napus (Brassica napus L.), there were 23 subgenomes, 11 subgenomes and 12 subgenomes of C, and the HMA gene was inhomogeneously doubled in three species. The 50 genes belong to the three subfamilies of PStub 1B-1 / P1B-2T / P1B-4, and there are differences in the distribution of protein conserved sequences, the physicochemical properties of proteins, the gene structure and the cis-acting elements in the promoter region, etc. However, there were significant differences in tissue expression specificity among genes of the same subgroup. Bra00311010 BnaA10g06240DnaC09g28870D BnaC09g11230DnBnaC04g20290DnaA09g10950D and Bra027641 homologous genes to at HMA5 were found to be more mutated than at HMA5 in many ways, which may lead to the extension of these genes in bolting crops with different functions than at HMA5. The new variation types of GICCSME and GICCPSE found in the HMA gene of Brassica Genome are different from the GICC- T / S-1 SE functional loci in Arabidopsis, and may lead to new functional variation of the corresponding genes. This study provides a bioinformatics reference for the selection of specific metal ion transport HMA genes from the AC genome of Brassica, and provides important guidance for further analysis of the metal ion transport mechanism in Brassica plants involved by HMA genes.
【作者单位】：中国农业科学院油料作物研究所/农业部油料作物生物学与遗传育种重点实验室;
【基金】：国家自然科学基金(31470088)
【分类号】：Q943.2

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