白茶萎凋过程中儿茶素合成关键酶基因表达分析

发布时间：2018-06-15 14:06

本文选题：白茶 + 萎凋　；参考：《南方农业学报》2016年08期

【摘要】：【目的】研究白茶萎凋过程中茶多酚、儿茶素组分含量变化与其合成途径中关键酶基因的动态表达,为优化白茶的萎凋工艺提供参考依据。【方法】以不同萎凋阶段的白茶为原料,分别采用福林酚(Folin-Ciocalten)比色法和高效液相色谱法(HPLC)测定茶多酚、儿茶素组分含量的变化,并以实时荧光定量PCR检测白茶萎凋过程中与儿茶素物质代谢合成相关基因(PAL、C4H、CHS、CHI、F3'5'H、F3'H、F3H、DFR、LAR、ANS、ANR和UGGT)的相对表达量。【结果】白茶萎凋过程中茶多酚含量呈逐渐降低趋势,儿茶素组分中表儿茶素(EC)、没食子儿茶素(GC)、表没食子儿茶素(EGC)、表儿茶素没食子酸酯(ECG)含量整体上呈先上升后下降的变化趋势,且均在萎凋历时32 h出现最大值。实时荧光定量PCR检测结果表明,儿茶素生物合成途径关键酶基因PAL、C4H、CHI、F3H、F3'H、F3'5'H、DFR、LAR、ANR和UGGT均在萎凋历时32 h呈上调表达,CHS基因则随萎凋历时的增加呈下调表达趋势,ANS基因表达在整个萎凋过程中呈先上升后下降的变化趋势,在萎凋历时16 h达最大值。白茶萎凋过程中儿茶素合成途径关键基因表达水平具有调控儿茶素生物合成的作用。【结论】在白茶萎凋过程中,儿茶素生物合成途径关键酶基因PAL、C4H、F3H、F3'H、DFR、LAR、ANR的表达与儿茶素组分单体EC、GC、EGC的积累规律基本一致,其最大值均出现在萎凋历时32 h,因此在制茶时可适当缩短萎凋时长,以提高白茶品质。
[Abstract]:[objective] to study the changes of tea polyphenols and catechins during withering and the dynamic expression of key enzyme genes in its synthesis pathway. In order to optimize the withering process of white tea, the contents of polyphenols and catechins were determined by Folin-Ciocaltenin colorimetric method and HPLC- HPLC. [methods] the content of polyphenols and catechins in tea was determined by Folin-Ciocaltenin colorimetry with different withering stages, [methods] the content of tea polyphenols and catechins was determined by HPLC. Real-time quantitative PCR was used to detect the relative expression levels of the genes associated with catechin metabolism and synthesis in white tea during withering. The content of tea polyphenols decreased gradually during withering of white tea during withering. The contents of epigallocatechin (ECG), non-catechin (GCN), epigallocatechin (EGCN) and epigallocatechin gallate (ECG) in the components of catechins increased first and then decreased, and all of them showed the maximum value at 32 h after withering. The results of real-time fluorescence quantitative PCR showed that, The key enzyme gene of catechin biosynthesis pathway, PALX C4HN, F3H, F3, F3, F3, F3, F3, F3, F3, F3, F5, and UGGT, were up-regulated and up-expressed during withering for 32 h, but down-regulated with the increase of withering duration, and the expression of ans gene increased first and then decreased during the whole process of withering, and then decreased in the whole course of withering, and the expression of CHS gene was down-regulated with the increase of withering time, and the expression of ANR and UGGT were down-regulated. The maximum withering time was 16 h. The key gene expression level of catechin biosynthesis pathway plays an important role in the regulation of catechin biosynthesis. [conclusion] during the withering process of white tea, catechin biosynthesis can be regulated. The expression of the key enzyme gene of catechin biosynthesis pathway, PALX C4HN, F3H, F3H, DFRRNR, was basically consistent with the accumulation rule of catechin component monomer ECG C, and its maximum value appeared in the withering time of 32 h. Therefore, the withering time could be shortened properly in tea making in order to improve the quality of white tea.
【作者单位】：福建农林大学园艺学院/茶学福建省高等学校重点实验室;
【基金】：国家自然科学基金项目(31270735) 福建茶产业农技推广服务试点建设项目(KNJ-151001)
【分类号】：TS272.5

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