猪伪狂犬病毒Bartha-K61疫苗株和变异株主要免疫原性基因分析

发布时间：2018-06-15 14:27

本文选题：猪伪狂犬病 + Bartha-K株　；参考：《中国动物传染病学报》2017年04期

【摘要】：自2011年底以来,我国多个省份暴发了猪伪狂犬病,一些研究院所证实当前猪伪狂犬病毒流行株发生了一定程度的变异,疫苗不能提供完全的保护。为了在分子生物学水平上比较不同厂家猪伪狂犬病毒Bartha-K61疫苗株,以及与当前流行的猪伪狂犬病病毒变异株,在主要免疫原性蛋白之间的差异,选取了4个厂家的活疫苗Bartha-K61株,通过二代测序方法进行测序,对Bartha-K61株gB、gC和gD这3种主要免疫原性基因以及推导出的氨基酸序列与变异株之间进行同源性分析。结果显示,Bartha-K61疫苗株与变异株gB蛋白序列中有23处特征性的差异,同源性为96.3%~96.9%;gC蛋白序列中有25处特征性的差异,同源性为92.7%~93.1%;gD蛋白序列中有10处特征性的差异,同源性为96.8%~97.3%;以gB、gC和gD氨基酸序列建立的进化分析结果显示,Bartha-K61疫苗株与变异株分属两个不同进化分支。
[Abstract]:Since the end of 2011, pig pseudorabies has occurred in many provinces of China. Some research institutes have confirmed that the current epidemic strain of pseudorabies virus has changed to a certain extent, and the vaccine can not provide complete protection. In order to compare the difference between Bartha-K61 vaccine strain of pig pseudorabies virus (PPRV) from different manufacturers and the prevalent variant strain of PPRV on molecular biological level, four live vaccine strains Bartha-K61 were selected. The main immunogenicity genes of Bartha-K61 strain gBGC and GD and the deduced amino acid sequences were analyzed by the second generation sequencing method. The results showed that there were 23 characteristic differences in GB protein sequence between Bartha-K61 vaccine strain and mutant strain, the homology was 96.3% 96.9% gC protein sequence, and the homology was 92.7% 93.1% gd protein sequence, there were 10 characteristic differences in the sequence of GB protein of Bartha-K61 vaccine strain and mutant strain, the homology was 96.3%, 96.9% g C protein sequence and 92.7% 93.1% gd protein sequence respectively. The results of evolutionary analysis based on the amino acid sequences of gBGC and GD showed that Bartha-K61 vaccine strain and mutant strain belonged to two different evolutionary branches.
【作者单位】：厦门市动物疫病预防控制中心;
【基金】：厦门市科技计划项目(3502Z20154095)
【分类号】：S858.28

【相似文献】