野生种花生钙依赖蛋白激酶基因家族的生物信息学分析

发布时间：2018-06-16 14:25

本文选题：野生种花生 + 钙依赖蛋白激酶　；参考：《热带作物学报》2017年01期

【摘要】：Ca2+是植物中重要的第二信使,几乎介导了植物生长发育的全部反应。钙依赖蛋白激酶(CDPKs)是植物中重要的钙传感蛋白,在植物生命活动中扮演着重要角色。目前2个二倍体野生种花生全基因组序列已经公布,而由2个野生种杂交后形成的异源四倍体栽培种花生的全基因组序列还没有公布,野生种花生CDPKs的生物信息学分析结果可以为栽培种花生CDPKs的研究提供参考。本研究采用生物信息学方法和工具对2个野生种花生CDPKs基因家族的全基因组分布、基因结构、进化和理化性质等进行分析。结果表明:2个野生种花生全基因组均比对到35个CDPKs基因序列,野生种花生与拟南芥CDPKs基因结构类似,蛋白质结构具有典型的蛋白质激酶和EF手型结构域,2个野生种花生与其它物种在进化过程中CDPKs变异较小,可能作为钙传感蛋白在细胞内各个部位和细胞器中行使功能。本研究结果可为栽培种花生的CDPKs结构和功能研究提供参考。
[Abstract]:CA 2 is an important second messenger in plants, which mediates almost all the responses to plant growth and development. Calcium dependent protein kinase (CDPKs) is an important calcium sensing protein in plants and plays an important role in plant life. At present, the whole genome sequence of two diploid wild species peanut has been published, but the whole genome sequence of allotetraploid peanut has not been published. The bioinformatics analysis results of the wild peanut CDPKs can provide reference for the study of the cultivated peanut CDPKs. In this study, bioinformatics methods and tools were used to analyze the whole genome distribution, gene structure, evolution and physicochemical properties of CDPKs gene family of two wild species of peanut. The results showed that there were 35 CDPKs gene sequences in the whole genome of two wild species of peanut, and the structure of CDPKs gene in wild species was similar to that in Arabidopsis thaliana. The protein structure has typical protein kinase and EF hand domain. The variation of CDPKs between two wild species peanut and other species is relatively small during the evolution, which may act as a calcium sensing protein in various parts of the cell and organelles. The results of this study can be used as a reference for the study of the structure and function of CDPKs in peanut cultivars.
【作者单位】：中国热带农业科学院椰子研究所;海南省热带油料作物生物学重点实验室;广西农业科学院经济作物研究所;
【基金】：海南省自然科学基金项目(No.20163144)
【分类号】：S565.2

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