DNMT3B基因在肝细胞癌中的表达及其对肝癌细胞增殖和侵袭迁移的影响

发布时间：2018-06-17 11:38

本文选题：肝细胞癌 + DNA甲基转移酶b(DNMTB)　；参考：《西安交通大学学报(医学版)》2017年03期

【摘要】：目的探讨DNA甲基转移酶3b(DNMT3B)基因在肝细胞癌(HCC)组织中的表达及其对肝癌细胞增殖、侵袭迁移的影响及机制。方法收集46例肝癌患者的癌组织与对应癌旁组织,qRT-PCR法检测DNMT3B在肝癌组织及对应癌旁组织中的表达,并分析其与临床病理特征之间的关系;应用RNA干扰(RNAi)技术合成针对DNMT3B的siRNA并转染MHCC97-H细胞,qRT-PCR和Western blot检测相关基因mRNA和蛋白表达;MTT法检测细胞增殖能力,Transwell实验检测细胞侵袭和迁移能力。结果 46例肝癌患者中,DNMT3B基因在肝癌组织中的表达较对应癌旁组织升高者占73.91%,其高表达与肝癌的病理分化类型和肿瘤大小有关(P均0.05);在细胞水平,抑制DNMT3B基因表达使MHCC97-H细胞增殖及侵袭迁移能力降低;干扰DNMT3B基因可以增加抑癌相关基因RASSFA1、APC、MTSS1mRNA和蛋白表达水平。结论 DNMT3B与肝癌的进展密切相关,它可能通过调控下游抑癌基因甲基化水平影响其表达,从而抑制肝癌细胞的增殖及侵袭迁移能力。
[Abstract]:Objective to investigate the expression of DNA methyltransferase (DNMT3B) gene in human hepatocellular carcinoma (HCC) and its effect on the proliferation, invasion and migration of hepatocellular carcinoma (HCC). Methods the expression of DNMT3B in HCC tissues and adjacent tissues was detected by qRT-PCR, and the relationship between DNMT3B and clinicopathological features was analyzed. The siRNA targeting DNMT3B was synthesized by RNAi technique and transfected into MHCC97-H cells. The mRNA and protein expression of related genes were detected by Western blot. The ability of cell proliferation was detected by MTT and the ability of cell invasion and migration was detected by Transwell assay. Results in 46 patients with HCC, the expression of DNMT3B gene was higher in HCC tissues than that in corresponding adjacent tissues (73.91%). The high expression of DNMT3B gene was related to the pathological differentiation type and tumor size of HCC. Inhibition of DNMT3B gene expression decreased the proliferation, invasion and migration of MHCC97-H cells, and increased the expression of RASSFA1APCMTSS1 mRNA and protein in MHCC97-H cells by interfering with DNMT3B gene. Conclusion DNMT3B is closely related to the progress of HCC. It may affect the expression of DNMT3B by regulating the methylation level of downstream tumor suppressor gene, thus inhibiting the proliferation, invasion and migration of HCC cells.
【作者单位】：西安交通大学第一附属医院消化内科;
【基金】：国家自然科学基金面上项目资助(No.30771895) 陕西省国际合作项目(No.2015KW-043)~~
【分类号】：R735.7

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