葡萄HSP17基因的合成与功能分析

发布时间：2018-06-17 12:46

本文选题：葡萄 + 小分子热激蛋白　；参考：《江苏农业学报》2017年03期

【摘要】：为了探究葡萄HSP17基因(Vv HSP17)的功能,以过量表达Vv HSP17的转基因拟南芥幼苗为材料,研究了高温胁迫对植株形态、叶绿素含量、丙二醛含量、脯氨酸含量、超氧化物歧化酶(SOD)活性的影响,同时还观察了苯酚胁迫对植株形态和种子萌发率的影响,并且比较了不同基因型植株对苯酚吸收降解能力的差异。结果表明,与野生型相比,转Vv HSP17基因拟南芥抗高温和苯酚胁迫的能力明显增强,逆境胁迫导致的叶绿素含量下降较少,丙二醛含量增幅较小,脯氨酸含量大幅升高,SOD酶活性较高,种子萌发率较高,处理液中苯酚残留量少。说明转Vv HSP17基因拟南芥对物理性高温胁迫和化学性苯酚胁迫都有较高的耐受性。
[Abstract]:In order to investigate the function of Vv HSP17 gene in grape, transgenic Arabidopsis thaliana seedlings which overexpressed Vv HSP17 were used to study the effects of high temperature stress on plant morphology, chlorophyll content, malondialdehyde content and proline content. Effects of superoxide dismutase (SOD) activity and phenol-stress on plant morphology and seed germination rate were also observed, and the differences in phenol uptake and degradation of different genotypes were compared. The results showed that compared with the wild type, the ability of transgenic Arabidopsis thaliana to resist high temperature and phenol stress was significantly enhanced, the chlorophyll content decreased less and the malondialdehyde content increased slightly under stress. The content of proline increased significantly, the activity of SOD was higher, the rate of seed germination was higher, and the residual amount of phenol in the treatment solution was less. The results indicated that Arabidopsis thaliana with Vv HSP17 gene had high tolerance to physical high temperature stress and chemical phenol stress.
【作者单位】：南京农业大学园艺学院;上海市农业科学院生物技术研究所;
【基金】：国家自然科学基金项目(31401820) 江苏省自然科学基金项目(BK20140702) 江苏省苏北科技发展计划项目(BN2012035)
【分类号】：S663.1

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