三七总皂苷对脑出血大鼠神经细胞凋亡及相关基因的影响

发布时间：2018-06-18 01:51

本文选题：三七总皂苷 + 脑出血　；参考：《暨南大学学报(自然科学与医学版)》2017年05期

【摘要】：目的:研究三七总皂苷对脑出血大鼠神经细胞凋亡及相关基因的影响.方法:将大鼠随机分为假手术组、模型组和三七总皂苷组,利用胶原酶注射尾状核法制作脑出血模型,利用免疫组化染色法及细胞凋亡染色法检测各组大鼠Bcl-2、Bax、Caspase-3及TUNEL阳性细胞数目.结果:在6 h、2 d、3 d、5 d、7 d共5个时间点,模型组、三七总皂苷组大鼠神经细胞凋亡情况、Bcl-2、Bax、Caspase-3与假手术组相比差异均显著,具有统计学意义(P0.05);三七总皂苷组大鼠神经细胞凋亡情况、Bcl-2、Bax、Caspase-3与模型组相比差异均显著,具有统计学意义(P0.05).结论:三七总皂苷治疗可以显著下调Bax、Caspase-3的表达水平,提高Bcl-2的表达水平,减少神经细胞凋亡,降低脑出血后继发性损伤.
[Abstract]:Aim: to study the effect of Panax notoginseng saponins on neuronal apoptosis and related genes in rats with intracerebral hemorrhage. Methods: rats were randomly divided into sham-operation group, model group and panax notoginseng saponins group. Intracerebral hemorrhage model was made by injection of collagenase into caudate nucleus. Immunohistochemical staining and apoptosis staining were used to detect the number of Caspase-3 and Tunel positive cells. Results: the apoptosis of nerve cells in model group, panax notoginseng saponins group and sham operation group were significantly different from those in sham operation group at 6 h, 2 d, 3 d, 5 d and 7 d, and the apoptosis of nerve cells in model group and panax notoginseng saponins group were significantly higher than those in sham operation group. The apoptosis of nerve cells in the panax notoginseng saponins group was significantly different from that in the model group. Conclusion: panax notoginseng saponins can significantly down-regulate the expression of Caspase-3, increase the expression of Bcl-2, reduce neuronal apoptosis and reduce the secondary injury after intracerebral hemorrhage.
【作者单位】：广东药科大学药学院;暨南大学附属第一医院药学部;
【基金】：广东省自然科学基金项目(2015A030313580)
【分类号】：R285.5

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