蝴蝶兰PhRCAβ基因的克隆及表达特性分析

发布时间：2018-06-19 00:42

  本文选题：蝴蝶兰 + 低温胁迫　； 参考：《基因组学与应用生物学》2017年06期

【摘要】：为研究蝴蝶兰Rubisco活化酶(RCA)基因对低温胁迫的响应机制,以蝴蝶兰"满天红"为材料,分析了蝴蝶兰Rubisco活化酶基因PhRCAβ的全长序列(Gen Bank登录号为KU954548)及其表达特性。结果表明,PhRCAβ基因全长1 695 bp,编码440个氨基酸;其编码的蛋白具有RCA的特异位点,含叶绿体转运肽,定位于叶绿体中,其成熟蛋白的分子量为42.52 k D,等电点为蛋白5.54,属于RCA小亚基基因;该基因在绿色组织中转录表达;13℃/8℃(昼/夜)的低温胁迫抑制Ph RCAβ基因的转录表达,并随着低温胁迫时间的延长,PhRCAβ基因的表达水平逐渐降低,在温度恢复正常时其表达水平升高;4℃低温条件下,Ph RCAβ基因的表达水平随着低温处理时间的延长逐渐降低,并一直维持在较低水平。由此推测,PhRCAβ直接参与了蝴蝶兰叶片光合作用的调控。
[Abstract]:In order to study the response mechanism of Rubisco activase (RCA) gene of Phalaenopsis to low temperature stress, the full-length sequence of Rubisco activase gene PhRCA 尾 of Phalaenopsis, named KU954548, was analyzed. The results showed that the full length of PhRCA 尾 gene was 1 695 BP, encoding 440 amino acids, and the encoded protein had the specific site of RCA, which contained chloroplast transporter peptide and was located in chloroplast. The molecular weight of the mature protein is 42.52 KD, the isoelectric point is 5.54, which belongs to the RCA small subunit gene, and the low temperature stress of 13 鈩，

本文编号：2037546