棉花中HD-Zip Ⅳ家族基因的全基因组鉴定及特征分析

发布时间：2018-06-19 08:33

本文选题：棉花 + HD-Zip转录因子　；参考：《分子植物育种》2017年08期

【摘要】：同源异型亮氨酸拉链蛋白(homeodomain-leucine zipper,HD-Zip)是高等植物特有的一类转录因子,分成Ⅰ~Ⅳ4个亚家族。HD-ZipⅣ转录因子主要参与表皮结构的发育调控,在其他作物中已经广泛研究,但是棉花中还未进行系统的分析。本研究中,我们分别对陆地棉、亚洲棉、雷蒙德氏棉进行全基因组的鉴定,分别得到15个、12个、14个HD-ZipⅣ基因。除一个雷蒙德氏棉基因发生丢失外,陆地棉中几乎保留了所有的亚洲棉、雷蒙德氏棉中的HD-ZipⅣ基因。分析表明这些基因编码的蛋白都有HD-ZipⅣ转录因子特征性的结构域,且进化树上关系近的成员拥有相似的基因结构和基序组成。大部分陆地棉HD-ZipⅣ基因经历了纯化选择,且有一些经历正向选择。同时,进一步分析得出结论:片段复制在陆地棉HD-ZipⅣ基因的演化中起重要作用。组织表达分析表明大部分HD-ZipⅣ基因主要在发育着的胚珠和纤维中表达,暗示该家族与棉花中种子形成、纤维发育有关。总之,本研究为棉花中HD-ZipⅣ基因的功能研究及应用提供了参考依据。
[Abstract]:Homologous homeodomain-leucine zipper (HD-Zip) is a specific transcription factor of higher plants. It is divided into 4 subfamilies, I ~ IV,.HD-Zip IV transcription factors, which are mainly involved in the development and regulation of epidermal structure. It has been widely studied in other crops, but it has not been systematically analyzed in cotton. In this study, we 15, 12, and 14 HD-Zip IV genes were identified for land cotton, Asian cotton and Raymond's cotton respectively. In addition to the loss of a Raymond's cotton gene, almost all of the Asian cotton and the HD-Zip IV gene in Raymond S cotton were retained in the land cotton. The analysis showed that all the genes encoded by these genes were HD-Zip (IV) the characteristic domain of the transcription factor, and the members of the evolutionary tree have similar genetic structure and motif. Most of the HD-Zip IV gene of upland cotton has been selected and has some positive selection. Tissue expression analysis showed that most of HD-Zip IV genes were expressed mainly in the developed ovules and fibers, suggesting that the family was related to the formation of seeds and fiber development in cotton. In a word, this study provides a reference for the function research and application of HD-Zip IV gene in cotton.
【作者单位】：河南农业大学农学院;中国农业科学院棉花研究所郑州科研中心棉花生物学国家重点实验室;
【基金】：国家自然基金项目(U1204312) 河南省棉花产业技术体系建设专项(S2013-07-G04) 郑州市科技计划项目(153PXXCY180)共同资助
【分类号】：Q943.2;S562

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