拟南芥类结瘤素基因At1g01070的生物信息学分析

发布时间：2018-06-19 06:51

本文选题：类结瘤素基因 + Atg　；参考：《河南师范大学学报(自然科学版)》2017年03期

【摘要】：类结瘤素基因在非结瘤植物生长发育中承担着重要的功能,但仅少数基因的功能被鉴定.本研究对拟南芥类结瘤素MtN21(Medicago truncatula NODULIN 21)家族成员At1g01070进行了生物信息学分析及亚细胞定位验证.结果表明,At1g01070有两种可变剪切体,编码两种蛋白At1g01070.1和At1g01070.2,前者比后者在N端多47个氨基酸,它们的分子量分别为40KD和35KD,等电点分别为9.2和8.9;均含有2个MtN21蛋白的特征结构域EamA(药物/代谢物转运结构域),At1g01070.1比At1g01070.2多一个PLN00411结构域,且其靠近N端的EamA结构域较后者长;三级结构均由7个α-螺旋和一些不规则卷曲组成,分别含有10个和8个疏水跨膜区,均含13个磷酸化修饰位点,亚细胞定位预测主要定位在质膜;进化上,与琴叶拟南芥(Arabidopsis lyrata)亲缘关系最近;亚细胞定位结果显示At1g01070.1定位在质膜,与预测结果一致.推测At1g01070在植物体内可能参与物质运输.
[Abstract]:The tumor like nodule gene plays an important role in the growth and development of non nodule plants, but only a few genes have been identified. Bioinformatics analysis and subcellular localization of At1g01070 MtN21 (Medicago truncatula NODULIN 21) family members of Arabidopsis thaliana have been tested in this study. The results show that there are two kinds of variable scissors in At1g01070. It encodes two kinds of protein At1g01070.1 and At1g01070.2, the former is 47 more amino acids than the latter at the N end, and their molecular weights are 40KD and 35KD respectively, and the isoelectric points are 9.2 and 8.9, respectively, which contain the characteristic domain EamA (drug / metabolite transport domain) of 2 MtN21 proteins, and At1g01070.1 is more than At1g01070.2 in a PLN00411 domain. The EamA domain in the near N end is longer than that of the latter; the three structure is composed of 7 alpha helix and some irregular curls, which contain 10 and 8 hydrophobic transmembrane regions, both containing 13 phosphorylation sites, and the subcellular localization prediction is mainly located in the plasma membrane; evolution, the closest relationship with the Arabidopsis lyrata; subcellular localization junction The results showed that At1g01070.1 was located in the plasma membrane and was consistent with the predicted results. It is speculated that At1g01070 may be involved in material transport in plants.
【作者单位】：河南师范大学生命科学学院;
【基金】：国家自然科学基金(31601241) 河南省高校科技创新团队(15IRTSTHN020) 河南师范大学博士科研启动基金(39970774)
【分类号】：Q943.2

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