横断山地区蘑菇属Minores组的多基因系统发育研究

发布时间：2018-06-19 05:19

本文选题：蘑菇科 + 系统发育学　；参考：《西南林业大学》2016年硕士论文

【摘要】：蘑菇属是伞菌目中的重要类群。其拥有极为丰富的物种,而且很多都具有重要的研究价值。最近研究发现该属具有5个亚属,20个组。本次研究以该属Minores组为研究对象,对82份标本进行系统发育学研究。运用形态学研究方法,将供试标本进行详尽的形态学观察,对观察结果进行详尽的描述以及绘图。运用分子系统学研究方法,对供试样本进行DNA的提取,PCR的扩增。扩增基因包括ITS、LSU、PRB2、tef1-α,共得到101条ITS,90条LSU,85条tef1-α和84条rpb2,从GenBank下载得到该组基因130条ITS,80条LSU,70条tef1-α和7条rpb2,。综合4个基因数据,运用最大似然法(ML)、最大简约法(MP)以及贝叶斯法(Bayes)进行系统发育树的构建。分子系统发育研究所得该组共14个支序,3个支序得到很好的支持,其余支序种间关系均得到很好支持。分析Minores组共66个种,其中17个拟定新种,4个中国新纪录种,13个未定种。对17个新种进行形态学描述,绘制相关形态图17份,对整个组的系统发育关系进行初步研究。
[Abstract]:The genus Agaricus is an important group in Umbellidae. It has extremely rich species, and many of them have important research value. Recently, it was found that the genus has 5 subgenera and 20 groups. In this study, 82 specimens of Minores group were studied on phylogeny. Using morphological research method, the specimen was observed in detail, and the observation results were described and plotted in detail. Molecular systematics method was used to amplify DNA extracted by PCR. The amplified genes included ITSN LSUB2PRB2 tef1- 伪, 101 ITSs, 90 LSUF1- 伪 and 84 rpb2s, and 130 ITSs from GenBank were downloaded from GenBank, and 70 LSUF1- 伪 and 7 rpb2s were obtained from GenBank. The phylogenetic tree was constructed by maximum likelihood method (MLN), maximum minimization method (MPS) and Bayesian method (Bayes). The molecular phylogeny study showed that there were 14 branches in this group, 3 of them were well supported, and all the other branch species were well supported. There are 66 species in Minores formation, including 17 new species, 4 new record species and 13 undetermined species. The phylogenetic relationships of 17 new species and 17 related morphological maps were studied.
【学位授予单位】：西南林业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：Q949.32

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