转Bt基因大米与相应亲本大米差异蛋白质组学研究

发布时间：2018-06-19 17:24

本文选题：转基因大米 + 差异蛋白质组学　；参考：《国际药学研究杂志》2016年04期

【摘要】：目的利用差异蛋白质组学及生物信息学等技术方法,探讨外源Bt基因的导入对大米表达蛋白质组的影响,深化转基因大米在蛋白质组学方面的研究。方法在转基因大米"华恢1号"和亲本大米"明恢63"样品中提取大米总蛋白,通过二维聚丙烯酰胺凝胶电泳(2D-PAGE)实验方法,得到相应的蛋白质组2D-PAGE谱,再选择差异明显的蛋白质点进行质谱鉴定及生物信息学分析。结果对转Bt(cry1Ab/1Ac)基因大米和亲本大米2D-PAGE图谱的蛋白质点进行了对比匹配,识别出明显的差异蛋白质点28个,以亲本大米为参照,转Bt基因大米相对高表达的18个,相对低表达的10个;选择转基因大米凝胶上的差异蛋白质点进行了质谱鉴定和生物信息学检索,发现差异蛋白质主要参与能量代谢,蛋白质合成、氧化还原和应激响应等生物过程。结论转Bt基因"华恢1号"及其亲本大米"明恢63"表达的蛋白质组存在一定差异,但未发现这些差异蛋白质具有抗营养性和致敏性,也未发现新蛋白和毒蛋白的表达。
[Abstract]:Objective to investigate the effect of exogenous BT gene introduction on the expression of proteome in rice by using differential proteomics and bioinformatics, and to deepen the proteomics of transgenic rice. Methods Total protein of rice was extracted from transgenic rice "Huanghui 1" and parent rice "Minghui 63", and the corresponding proteome 2D-PAGE was obtained by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Protein spots with obvious difference were selected for mass spectrometry identification and bioinformatics analysis. Results the protein spots in the 2D-PAGE map of transgenic BT cry1Ab / 1Acc gene rice and parental rice were compared and matched. 28 distinct differentially expressed protein spots were identified, 18 of which were relatively overexpressed in transgenic BT rice, compared with parental rice. The differential protein spots on the gels of transgenic rice were identified by mass spectrometry and bioinformatics search. It was found that differential proteins were mainly involved in energy metabolism and protein synthesis. Biological processes such as redox and stress response. Conclusion the proteome of transgenic BT gene "Huanghui 1" and its parent rice "Minghui 63" has some differences, but these proteins have not been found to be nutritionally resistant and sensitized, nor have the expression of new protein and toxin protein been found.
【作者单位】：国家生物医学分析中心;
【基金】：转基因生物新品种培育国家科技重大专项资助项目(2014ZX08011007) 国家重大科学仪器设备开发专项资助项目(2012YQ180117)
【分类号】：S511

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