滇牡丹类黄酮3-O-葡萄糖基转移酶基因的鉴定及表达分析

发布时间：2018-06-19 18:08

本文选题：滇牡丹 + GT　；参考：《基因组学与应用生物学》2017年04期

【摘要】：在植物中,类黄酮3-O-葡萄糖基转移酶(F3GT)将不稳定的花色素转变为稳定的花色素苷,是花色素苷形成的关键酶。本研究采样RT-PCR技术从滇牡丹中成功克隆获得一个完整的F3GT基因(Pd F3GT1),其含有1 371 bp的开放阅读框(ORF),编码456个氨基酸。该基因推断的蛋白与葡萄(Vitis vinifera)3GT蛋白的相似性为64%;与已知功能的3GT基因进行系统进化分析,结果显示Pd3GT1与葡萄3GT聚类到同一个分支。氨基酸比对显示Pd3GT1蛋白序列还有糖基转移酶家族特有的PSPG-box结构域。半定量PCR结果显示:Pd3GT1在花蕾,花开时期的花瓣中大量表达,在叶和花芽中有微弱表达;在根和茎中没有表达。说明Pd3GT基因表达具有组织表达特异性。该研究将为研究滇牡丹花色素形成机理,以及对滇牡丹类黄酮糖基转移酶的功能鉴定和利用基因工程手段改良花卉品质提供理论依据。
[Abstract]:In plants, flavonoid 3-O- glucosyltransferase F3GT) converts unstable anthocyanins into stable anthocyanins, which is the key enzyme for anthocyanin formation. In this study, a complete F3GT gene, Pd-F3GT1, was successfully cloned from Peony by sampling RT-PCR, which contained an open reading frame of 1 371 BP, encoding 456 amino acids. The similarity between the deduced protein and Vitis vinifera 3GT protein was 64, and the phylogenetic analysis showed that Pd3GT1 and grape 3GT were clustered into the same branch. Amino acid alignment showed that the Pd3GT1 protein sequence and the PSPG-box domain of the glycosyltransferase family. The results of semi-quantitative PCR showed that Pd3GT1 was strongly expressed in flower buds and petals at flowering stage, but weakly in leaves and flower buds, but not in roots and stems. The results showed that the expression of PD 3 GT gene had tissue expression specificity. This study will provide a theoretical basis for the study of the formation mechanism of pigment, the function identification of flavonoid glycosyltransferase and the improvement of flower quality by genetic engineering.
【作者单位】：云南林业学科院云南省森林植物培育与开发利用重点实验室;云南林业学科院国家林业局云南珍稀濒特森林植物繁育和保护重点实验室;西南林业大学;
【基金】：云南省林业科学院重点实验室自主创新项目(22CX2016-05) 云南省科技计划项目(2015A005) 云南省应用基础研究重点项目(2013FA054)共同资助
【分类号】：Q943.2;S685.11

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