通关藤提取物诱导BGC-823细胞凋亡及对MTDH基因表达的作用研究

发布时间：2018-06-20 06:08

本文选题：通关藤提取物 + BGC-　；参考：《中华中医药学刊》2017年04期

【摘要】：目的:通过观察不同浓度通关藤提取物对人胃腺癌BGC-823细胞增殖、凋亡及MTDH基因表达的影响,为胃癌的临床治疗提供实验基础及潜在治疗靶点。方法:(1)MTT法检测通关藤提取物对BGC-823细胞增殖影响。(2)Annexin V/PI双染法检测通关藤提取物对BGC-823细胞凋亡影响。(3)反转录多聚酶链反应(RTPCR)检测通关藤提取物对BGC-823细胞MTDH mRNA表达影响。(4)Western Blot法检测通关藤提取物对BGC-823细胞凋亡相关蛋白表达影响。结果:(1)通关藤提取物作用于BGC-823细胞,细胞生长抑制率随药物浓度增大而升高,加药各组与对照组相比,差异有统计学意义。(2)通关藤提取物0 mmol/L、0.5 mmol/L、1 mmol/L、2mmol/L诱导BGC-823细胞凋亡率为(2.98±1.01)%、(6.79±0.68)、(12.512±1.27)%、(16.97±1.09),与对照组相比差异有统计学意义。(3)通关藤提取物可下调BGC-823细胞MTDH mRNA表达,这种作用具有浓度依赖性。(4)通关藤提取物能够下调Bcl-2家族中的抑凋亡因子Bcl-2、Bcl-xl的表达尤其是Bcl-xl的表达,上调凋亡执行者Caspase-3的表达。结论:通关藤提取物可下调BGC-823细胞MTDH mRNA表达,并通过下调Bcl-2、Bcl-xl,上调Caspase-3的表达而诱导BGC-823细胞凋亡,从而抑制了BGC-823细胞的生长增殖。
[Abstract]:Aim: to investigate the effects of different concentrations of Tungkuan extract on the proliferation, apoptosis and MTDH gene expression of human gastric adenocarcinoma cell line BGC-823 in order to provide experimental basis and potential therapeutic target for the clinical treatment of gastric cancer. Methods MTT assay was used to detect the proliferation of BGC-823 cells. The effect of Annexin V / Pi double staining on the apoptosis of BGC-823 cells was detected by MTT) reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of MTDH mRNA in BGC-823 cells by reverse transcription polymerase chain reaction (RT-PCR). The expression of apoptosis-related protein in BGC-823 cells was detected by Western blot. Results the growth inhibition rate of BGC-823 cells increased with the increase of drug concentration. The apoptosis rate of BGC-823 cells induced by 0 mmol / L 0 mmol / L ~ (0.5) mmol / L ~ (1) mmol / L ~ (2) / L was 2.98 卤1.01% (P < 0.05). The expression of MTDH mRNA in BGC-823 cells was significantly lower than that in the control group (P < 0.05), and the expression of MTDH mRNA in BGC-823 cells was significantly lower than that in the control group (P < 0.05), and the expression of MTDH mRNA in BGC-823 cells was significantly lower than that in the control group (P < 0.05), and the expression of MTDH mRNA in BGC-823 cells was significantly lower than that in control group. This effect was concentration-dependent. The extract of Tongguan vine could down-regulate the expression of Bcl-2 family inhibitor Bcl-2nBcl-xl, especially the expression of Bcl-xl, and up-regulate the expression of Caspase-3 in apoptotic executors. Conclusion: the extract of Tongguan vine can down-regulate the expression of MTDH mRNA in BGC-823 cells and inhibit the growth and proliferation of BGC-823 cells by down-regulating the expression of Bcl-2OBcl-xland up-regulating the expression of Caspase-3 in BGC-823 cells.
【作者单位】：辽宁中医药大学;辽宁中医药大学附属第二医院;沈阳军区总医院;
【基金】：国家“十二五”重大新药创制平台子课题项目(2012ZX-09303016-002) 辽宁省科技攻关计划项目(2012225019) 辽宁省中医药临床专(学)科能力建设项目
【分类号】：R285

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