GPR56基因在骨肉瘤组织中的表达变化及其对骨肉瘤细胞增殖、迁移、侵袭能力的影响

发布时间：2018-06-20 07:16

本文选题：骨肉瘤 + G蛋白偶联受体　；参考：《山东医药》2017年32期

【摘要】：目的探讨G蛋白偶联受体56(GPR56)基因在骨肉瘤发生、发展中的作用。方法选取拟行手术治疗的骨肉瘤患者53例,术中留取骨肉瘤组织及其癌旁正常组织,采用实时荧光定量PCR法检测组织GPR56 mRNA相对表达量,并分析其与患者临床病理参数的关系。将对数生长期人骨肉瘤细胞株MG-63随机分为三组,其中siRNA-GPR56组转染GPR56干扰序列,siRNA-对照组转染siRNA对照序列,空白对照组不作任何处理。采用实时荧光定量PCR法检测各组细胞GPR56 mRNA相对表达量,CCK-8法和克隆形成试验检测细胞增殖能力(分别以培养24、48、72、96 h的吸光度值及克隆形成率表示),Transwell法检测细胞迁移、侵袭能力(以迁移、侵袭细胞数表示)。结果骨肉瘤组织GPR56 mRNA相对表达量为1.86±0.14,癌旁正常组织为1.39±0.12,二者比较P0.01。骨肉瘤组织GPR56 mRNA相对表达量与患者年龄、性别、发病部位、肿瘤直径、组织类型、血清碱性磷酸酶水平均无关(P均0.05),与Enneking分期、肺部转移、软组织浸润均有关(P均0.05)。siRNA-GPR56组GPR56mRNA相对表达量明显低于siRNA-对照组及空白对照组(P均0.01)。siRNA-GPR56组培养48、72、96 h吸光度值及克隆形成率、迁移细胞数、侵袭细胞数均低于siRNA-对照组及空白对照组(P0.05或0.01)。结论骨肉瘤组织中GPR56基因表达升高,GPR56基因可能通过影响细胞增殖、迁移和侵袭能力而参与骨肉瘤的发生与发展。
[Abstract]:Objective to investigate the role of G protein coupled receptor 56 (GPR56) gene in the pathogenesis and development of osteosarcoma. Methods A total of 53 patients with osteosarcoma were selected. The tissues of osteosarcoma and its adjacent normal tissues were collected during the operation. The relative expression of GPR56 mRNA was detected by real-time fluorescence quantitative PCR, and the relationship between GPR56 mRNA expression and clinicopathological parameters was analyzed. Human osteosarcoma cell line MG-63 was randomly divided into three groups: siRNA-GPR56 group transfected with GPR56 interference sequence and control group transfected with siRNA control sequence. The relative expression of GPR56 mRNA was detected by real-time fluorescence quantitative PCR and the cell proliferation was detected by CCK-8 assay and clone formation assay. Invasive ability (expressed by migration, number of invading cells). Results the relative expression of GPR56 mRNA was 1.86 卤0.14 in osteosarcoma and 1.39 卤0.12 in adjacent normal tissue. The relative expression of GPR56 mRNA in osteosarcoma was not correlated with age, sex, location, tumor diameter, tissue type, serum alkaline phosphatase level, but with Enneking stage and lung metastasis. The relative expression of GPR56 mRNA in group P was significantly lower than that in group siRNA.siRNA-GPR56. The relative expression of GPR56 mRNA was significantly lower than that of control group and blank control group (P < 0.01). SiRNA-GPR56 group was cultured for 48 h, the absorbance value and colony formation rate, the number of migrating cells, and the relative expression of GPR56 mRNA were significantly lower than those in control group and blank control group. The number of invasive cells was lower than that of siRNA-control group and blank control group (P0.05 or 0.01). Conclusion the increased expression of GPR56 gene in osteosarcoma may be involved in the development of osteosarcoma by affecting cell proliferation, migration and invasion.
【作者单位】：潍坊市益都中心医院;
【分类号】：R738.1

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