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耐弱光基因型马铃薯在遮阴条件下的光合和荧光特性分析

发布时间:2018-06-22 04:35

  本文选题:马铃薯 + 耐弱光 ; 参考:《中国生态农业学报》2017年08期


【摘要】:为探寻遮阴对马铃薯光合和荧光特性及吸收光能分配的影响,本研究采用大田试验,以马铃薯不耐弱光品种‘丽薯6号’和耐弱光品种‘中薯20’为材料,出苗后用遮光率70%的黑色遮阳网进行遮阴处理(T),以自然光照为对照(CK),测定了遮阴30 d后马铃薯叶片的光合作用、光响应曲线、CO_2响应曲线、光诱导曲线和叶绿素荧光参数的变化。结果表明:1)遮阴后净光合速率(P_n)、气孔导度(G_s)、蒸腾速率(T_r)、最大净光合速率(P_(max))、光饱和点(LSP)、光补偿点(LCP)较CK显著下降;‘中薯20’的P_n、P_(max)、LSP较高,LCP和暗呼吸速率(R_d)较低。2)不同基因型CO_2响应参数无显著差异,但‘中薯20’的最大羧化速率(V_(cmax))和最大电子传递速率(Jmax)较高,CO_2补偿点(CCP)较低。3)高光诱导过程中,‘中薯20’反应较快,光合能力较强。4)初始荧光(F_o)、最大荧光(F_m)、最大光化学量子效率(F_v/F_m)较CK显著增加,PSⅡ实际光化学量子效率(?F/F_m′)、表观电子传递速率(ETR)和光化学猝灭系数(qP)较CK显著下降,非光化学猝灭系数(NPQ)呈增加趋势。5)非光化学热耗散(ФNPQ)和荧光耗散途径(Фf,d)比例显著增加,光化学猝灭耗散途径(ФPSⅡ)比例显著减少,主要以增加热耗散为主。遮阴后,耐弱光基因型‘中薯20’的NPQ和ФNPQ均高于不耐弱光的‘丽薯6号’,说明‘中薯20’的光合机构保护能力更强。综合分析表明,遮阴后耐弱光基因型马铃薯具有较高的净光合速率、较低的光补偿点、较低的CO_2补偿点、较快的光诱导反应速度和较高的非光化学热耗散能力。
[Abstract]:In order to explore the effect of shading on photosynthetic and fluorescence characteristics and absorption energy distribution of potato, the field experiment was carried out in this paper. The potato varieties' Lishu 6 'and' Zhongshu 20 'were used as the materials, and the two varieties,' Lishu 6 'and' Zhongshu 20', were used as materials. After seedling emergence, shading treatment (T) was carried out with a black sunshade net with 70% shading rate. The photosynthesis, photoresponse curve, photo-induced curve and chlorophyll fluorescence parameters of potato leaves after 30 days shading were measured by using natural light as control (CK). The results showed that net photosynthetic rate (PN), stomatal conductance, transpiration rate, maximum net photosynthetic rate (P _ (max), light saturation point) and light compensation point (LCP) were significantly decreased after shading. There was no significant difference in CO2 response parameters among different genotypes. However, the maximum carboxylation rate (V _ (cmax) and maximum electron transfer rate (Jmax) of 'Zhongshu 20' was higher than that of Cost2 compensation point (CCP). 3) in the process of high light induction, the reaction of 'Zhongshu 20' was faster. Compared with CK, the maximum photochemical quantum efficiency (FV / FM), the apparent electron transfer rate (ETR) and the photochemical quenching coefficient (QP) of PS 鈪,

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