导入OsVTE基因提高普那菊苣的抗旱性

发布时间：2018-06-22 18:03

本文选题：维生素E(OsVTE) + 普那菊苣　；参考：《草地学报》2017年04期

【摘要】：通过植物基因工程手段培育转基因作物品种,可改善作物的生存能力和对生态环境的耐受力。本研究以普那菊苣叶片(Cichorium intybus L.‘Puna’)为受体材料,用农杆菌介导方法,将来自水稻的由CaMV35S启动子驱动的生育酚环化酶(tocopherol cyclase,OsVTE)基因导入普那菊苣。经过卡那霉素(Km)筛选以及对抗性植株PCR和Southern杂交分析,获得了转基因植株。用不同浓度的蔗糖、琼脂和甘露醇溶液研究普那菊苣幼苗的耐旱性,发现转基因植株的耐旱性明显得到提高。蔗糖浓度为50g·L~(-1)、琼脂浓度为10g·L~(-1)和甘露醇浓度为30g·L~(-1)胁迫下普那菊苣的生理指标测定显示,转基因普那菊苣幼苗SOD、POD活性比未转化植株高2~3倍,转基因普那菊苣幼苗MDA含量降低。本研究获得了稳定的转OsVTE基因植株,旱胁迫下的转基因植株的SOD酶活性和POD酶活性含量提高,表现出一定的耐旱性。
[Abstract]:Breeding transgenic crop varieties by means of plant genetic engineering can improve the survival ability of crops and their tolerance to ecological environment. In this study, tocopherol cyclase OsVTE, which was driven by CaMV35S promoter from rice, was introduced into Phuna chicory by Agrobacterium tumefaciens mediated by Cichorium intybus L. Puna'. Transgenic plants were obtained by kanamycin (km) screening, PCR and Southern blot analysis. The drought tolerance of Phuna chicory seedlings was studied with different concentrations of sucrose, Agar and mannitol solution, and it was found that the drought tolerance of transgenic plants was obviously improved. Under the stress of 50 g L ~ (-1) of sucrose, 10 g L ~ (-1) of Agar and 30 g L ~ (-1) of mannitol, the activity of POD in POD of transgenic Phuna chicory seedlings was 2 or 3 times higher than that of non-transformed plants, and the MDA content of transgenic seedlings was decreased. In this study, stable transgenic plants with OsVTE gene were obtained. The SOD activity and POD activity of transgenic plants under drought stress were increased, showing drought resistance.
【作者单位】：山西省农业科学院农作物品种资源研究所农业部黄土高原作物基因资源与种质创制重点实验室杂粮种质资源发掘与遗传改良山西省重点实验室;
【基金】：国家自然科学基金(31501372) 山西省农科院所长青年引导专项(yydzx02) 山西省农科院种业发展项目(2016zyzx41)资助
【分类号】：S548

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