长春花感染黄龙病菌多酚氧化酶基因的表达及活性分析

发布时间：2018-06-22 18:19

本文选题：长春花 + 黄龙病菌　；参考：《华南农业大学学报》2017年02期

【摘要】：【目的】分析长春花Catharanthus roseus受黄龙病菌Candidatus Liberibacter asiaticus侵染后多酚氧化酶(PPO)基因的表达及活性变化。【方法】根据不同作物多酚氧化酶基因PPO序列的相似性,筛选出长春花多酚氧化酶基因Pw PPO引物,PCR扩增得Pw PPO基因片段,运用qRT-PCR和生理生化方法分析长春花嫁接黄龙病芽后,不同时间长春花叶、主茎和根中Pw PPO基因的表达量和酶活性变化。【结果】长春花受黄龙病菌侵染后,叶、主茎和根中Pw PPO基因的表达分别在嫁接后30、35和40 d上调表达4.23、12.64和33.80倍,不同组织中Pw PPO基因上调表达的时间和幅度不同。染病长春花的叶、主茎和根中PPO活性分别在嫁接后30、35和45 d为对照的2.87、2.10和1.89倍。【结论】Pw PPO基因表达量与PPO活性成正比,与长春花抵抗黄龙病菌侵染的能力密切相关。
[Abstract]:[objective] to analyze the expression and activity of polyphenol oxidase (PPO) gene in Catharanthus roseus infected by Candidatus Liberibacter asiaticus. [methods] according to the similarity of polyphenol oxidase gene sequence in different crops, The polyphenoloxidase gene PwPPO gene fragment was amplified by PCR. The results were analyzed by qRT-PCR and physiological and biochemical methods. [results] the expression of Pw PPO gene in leaves, main stems and roots was increased by 4.23 ~ 12.64 and 33.80 times in leaves, main stems and roots on day 3035 and 40 after grafting, respectively. The time and amplitude of the up-regulation of the PPO gene were different in different tissues. The activity of PPO in the leaves, main stems and roots of the infected roseflowers was 2.87, 2.10 and 1.89 times of that of the control at 30 ~ 35 and 45 days after grafting, respectively. [conclusion] the expression of Pw PPO gene is directly proportional to the PPO activity, which is closely related to the ability of rosebush to resist the infection of Xanthosaurus roseus.
【作者单位】：广东海洋大学农学院;华南农业大学农学院/广东省微生物信号与作物病害防控重点试验室;
【分类号】：S828

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