8年生转基因库安托杨外源基因转移及对土壤微生物数量影响的检测

发布时间：2018-06-22 18:20

  本文选题：转多基因杨树 + 基因水平转移　； 参考：《林业科学研究》2017年02期

【摘要】：[目的]评价转基因库安托杨可能引起的生态风险。[方法]利用PCR扩增研究外源基因水平转移情况,利用稀释平板法研究土壤中细菌、真菌和放线菌数量。[结果]电泳结果显示:林下杂草混合样品和土壤微生物DNA样品中均未出现目的基因片段。转基因株系和非转基因株系的非根际土壤中可培养的细菌、真菌和放线菌数量在杨树的不同生长期出现一定的变化,但是这种变化没有明显的规律。[结论]8年生转基因库安托杨未出现外源基因水平转移,也未对土壤微生物的数量产生显著影响。
[Abstract]:[objective] to evaluate the ecological risk of transgenic Cuantuo poplar. [methods] the horizontal transfer of exogenous genes was studied by PCR amplification, and the number of bacteria, fungi and actinomycetes in soil was studied by dilution plate method. [results] Electrophoretic results showed that there were no target gene fragments in mixed weed samples and soil microbial DNA samples. The number of culturable bacteria, fungi and actinomycetes in non-rhizosphere soil of transgenic and non-transgenic strains changed in different growth stages of poplar, but there was no obvious change in this change. [conclusion] there was no horizontal transfer of exogenous gene and no significant effect on soil microorganism quantity in 8-year-old transgenic Cuantuo poplar.
【作者单位】： 林木遗传育种国家重点实验室国家林业局林木培育重点实验室中国林业科学研究院林业研究所;
【基金】：国家“863”国家高技术研究发展计划项目(2013AA102703) 林业公益性行业科研专项经费重大项目(201004004)
【分类号】：S792.11;S714.3
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