人参中苯甲酸胁迫响应基因WRKY7的克隆与表达分析

发布时间：2018-06-23 04:27

本文选题：人参 + WRKY转录因子　；参考：《中国中药杂志》2017年06期

【摘要】：WRKY转录因子是高等植物中最重要的转录因子基因家族之一,其在植物形态建成、生长发育以及应对生物(病菌、害虫等)、非生物(干旱、盐、冷、热等)胁迫过程中发挥重要作用。该研究根据人参化感自毒物质苯甲酸诱导的人参转录组数据,设计全长扩增引物,利用RT-PCR方法获得1条编码WRKY转录因子的基因,暂命名为WRKY7,并对其进行TA克隆、测序和序列分析。该基因cDNA全长1 216 bp,其开放阅读框(open reading frame,ORF)为1 014 bp,编码337个氨基酸。序列分析及系统进化分析表明,该蛋白属于WRKY家族第Ⅲ类成员,与西洋参种的WRKY6转录因子亲缘关系最近,同源性为87%。实时荧光定量PCR分析发现,苯甲酸胁迫后WRKY7的表达量显著升高,推测该基因可能参与了人参响应苯甲酸胁迫的过程,为进一步研究人参自毒胁迫响应的分子机制奠定了基础。
[Abstract]:WRKY transcription factor is one of the most important transcription factor gene families in higher plants, it is in plant morphogenesis, growth and development and response to biological (bacteria, pests, etc.), abiotic (drought, salt, cold, etc.), Heat) plays an important role in the process of stress. In this study, based on panax ginseng transcriptome data induced by benzoic acid, a novel gene encoding WRKY transcription factor was obtained by RT-PCR, which was tentatively named WRKY7 and cloned by TA. Sequencing and sequence analysis. The total length of the cDNA is 1 216 BP, and the open reading frame (open reading frame ORF is 1 014 BP, encoding 337 amino acids. Sequence analysis and phylogenetic analysis showed that the protein belonged to the third class member of WRKY family and had the closest relationship with WRKY6 transcription factor of Panax quinquefolium with a homology of 87g. Real-time fluorescence quantitative PCR analysis showed that the expression of WRKY7 was significantly increased after benzoic acid stress, which suggested that the gene might be involved in the process of ginseng response to benzoic acid stress, which laid a foundation for further study on the molecular mechanism of ginseng autotoxic stress response.
【作者单位】：中国医学科学院北京协和医学院药用植物研究所;
【基金】：国家自然科学基金项目(81373911) 中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-017)
【分类号】：S567.51

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