微小牛蜱P0基因原核表达及表达产物的免疫原性分析

发布时间：2018-06-23 08:09

本文选题：微小牛蜱 + P基因　；参考：《黑龙江畜牧兽医》2017年09期

【摘要】：为了研究微小牛蜱P0蛋白的免疫原性,试验采用RT-PCR技术从微小牛蜱中扩增P0基因,将PCR产物克隆连入PET28a(+)载体构建重组表达载体PET28a-P0,再将其转化到大肠杆菌BL21(DE3)感受态细胞中以IPTG诱导表达。结果表明:P0基因获得表达,表达的重组蛋白分子质量为35 ku,表达产物能被微小牛蜱阳性血清所识别。说明P0蛋白具有良好的免疫原性。
[Abstract]:In order to study the immunogenicity of P0 protein of bovine ticks, the P0 gene was amplified from bovine ticks by RT-PCR technology, and PCR product clones were cloned into PET28a (+) vector to construct a recombinant expression vector PET28a-P0, and then transformed into BL21 (DE3) receptive cells in Escherichia coli with IPTG induced expression. The results showed that the P0 gene was expressed and expressed. The recombinant protein has a molecular mass of 35 Ku, and the expressed product can be identified by the positive immunized serum of S. nixus, indicating that P0 protein has good immunogenicity.
【作者单位】：河北农业大学动物医学院;阜平县畜牧水产局;
【基金】：河北省现代农业产业技术体系奶牛产业创新团队建设项目(HBCT2013080204) 河北农业大学科研发展基金计划项目(1509003)
【分类号】：S852.746

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