苹果‘长富2号’开花调控转录因子基因MdIDD7的克隆及表达分析

发布时间：2018-06-25 01:23

本文选题：苹果 + 成花诱导　；参考：《园艺学报》2017年05期

【摘要】：以‘长富2号’苹果为试验材料,采用RT-PCR的方法,从其芽中克隆得到INDETERMINATE DOMAIN(IDD)转录因子基因MdIDD7,其开放阅读框长度为1 626 bp,编码541个氨基酸。序列比对和结构域分析表明,该转录因子含有1个核定位信号和4个高度保守的锌指蛋白结构域。系统进化树分析表明,MdIDD7与白梨(Pyrus×bretschneideri,XP_009364602.1)、桃(Prunus persica,XP_007225628.1)和梅(Prunus mume,XP_008220893.1)聚在一起。实时荧光定量PCR表明,MdIDD7在‘长富2号’不同组织(茎、叶、花、果和芽)中均有表达,其中芽的表达量最高。花后40~60 d,MdIDD7在易成花品种‘烟富6号’芽中表达量显著高于难成花品种‘长富2号’。"小年"树顶芽组织中MdIDD7的表达量显著高于"大年"树。在花芽诱导前期,外源GA处理诱导MdIDD7下调表达,而蔗糖处理诱导其上调表达,说明MdIDD7响应激素和糖信号,促进苹果成花。
[Abstract]:Using 'Changfu 2' apple as experimental material, the transcription factor gene MdIDD7 of INDETERMINATE Domain (IDD) was cloned from its bud by RT-PCR. The open reading frame length of MdIDD7 was 1 626 BP, encoding 541 amino acids. Sequence alignment and domain analysis showed that the transcription factor contained one nuclear localization signal and four highly conserved zinc finger protein domains. Phylogenetic tree analysis showed that MdIDD7 was clustered with white pears (Pyrus 脳 bretschneidern XP009364602.1), peach (Prunus persicaanus XP00725628.1) and Prunus mumemeth XP008220893.1. Real-time fluorescent quantitative PCR showed that MdIDD7 was expressed in different tissues (stem, leaf, flower, fruit and bud) of 'Changfu 2', and the highest expression was found in bud. The expression of MdIDD7 in the bud of 'Yanfu 6', an easy flower variety, was significantly higher than that of the difficult flower variety 'Changfu 2'. " The expression of MdIDD7 in terminal bud tissue of small year tree was significantly higher than that of big year tree. In the early stage of flower bud induction, exogenous GA treatment induced down-regulation of MdIDD7 expression, while sucrose treatment induced up-regulation of MdIDD7 expression, which indicated that MdIDD7 responded to hormone and sugar signals and promoted apple flower formation.
【作者单位】：西北农林科技大学园艺学院;
【基金】：国家现代农业产业技术体系项目(CARS-28) 国家苹果改良中心杨凌分中心,陕西省农业科技创新与攻关项目(2015NY114) 陕西省果业发展协同中心和陕西果业发展项目国家自然科学基金项目(31672101)
【分类号】：S661.1

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