有氧运动对兔骨关节炎软骨细胞凋亡及相关基因蛋白表达的影响

发布时间：2018-06-25 09:24

本文选题：兔 + 关节炎软骨细胞　；参考：《动物医学进展》2017年10期

【摘要】：探讨有氧运动对兔骨关节炎软骨细胞凋亡及Bcl-2、Bax、MMP-2、MMP-13、β-catenin、GSK-3β蛋白表达的影响。建立兔骨关节炎(OA)模型,试验分为空白对照组、模型对照组、低强度运动组、中等强度运动组。TUNEL标记法检测不同强度有氧运动对关节炎软骨细胞凋亡的影响;Western blot检测Bcl-2、Bax、MMP-2、MMP-13、β-catenin、GSK-3β蛋白表达。模型对照组、低强度运动组、中等强度运动组关节炎软骨细胞凋亡率均显著高于空白对照组,低强度运动组、中等强度运动组关节炎软骨细胞凋亡率均显著低于模型对照组,中等强度运动组关节炎软骨细胞凋亡率显著低于低强度运动组(P0.01);模型对照组、低强度运动组、中等强度运动组关节炎软骨细胞Bcl-2、MMP-2、MMP-13、β-catenin、GSK-3β蛋白表达均显著高于空白对照组,Bax蛋白表达显著低于空白对照组;低强度运动组、中等强度运动组关节炎软骨细胞Bcl-2、MMP-2、MMP-13、β-catenin、GSK-3β蛋白表达均显著低于模型组对照组,Bax蛋白表达显著高于模型对照组;中等强度运动组关节炎软骨细胞Bcl-2、MMP-2、MMP-13、β-catenin、GSK-3β蛋白表达均显著低于低强度运动组,Bax蛋白表达显著高于低强度运动组(P0.01)。不同强度有氧通过调节Bcl-2和Bax蛋白表达降低关节炎软骨细胞凋亡,并下调MMP-2、MMP-13,降低软骨细胞的损伤,其机制与下调Wnt/β-catenin信号通路有关。
[Abstract]:To investigate the effects of aerobic exercise on the apoptosis of chondrocytes and the expression of MMP-13, 尾 -cateninine GSK-3 尾 in osteoarthritis chondrocytes in rabbits. The model of rabbit osteoarthritis (OA) was established. The experiment was divided into three groups: blank control group, model control group, low intensity exercise group. Effects of aerobic exercise of different intensities on apoptosis of chondrocytes in patients with arthritic chondrocytes. Expression of MMP-13 and GSK-3 尾 protein in Bcl-2AX MMP-2MMP-2 and 尾 -cateninine were detected by Western blot in moderate intensity exercise group. The apoptotic rate of arthritis chondrocytes in model control group, low intensity exercise group and moderate intensity exercise group was significantly higher than that in blank control group, and the apoptotic rate of arthritis chondrocyte in low intensity exercise group and moderate intensity exercise group was significantly lower than that in model control group. The apoptosis rate of chondrocytes in moderate exercise group was significantly lower than that in low intensity exercise group (P0.01), while in model control group, low intensity exercise group, The expression of Bcl-2MMP _ 2 MMP-13, 尾 -cateninine GSK-3 尾 protein in arthritis chondrocytes in moderate exercise group was significantly higher than that in blank control group, and the expression of Bax protein in low-intensity exercise group was significantly lower than that in blank control group. The expression of Bcl-2MMP _ 2 MMP-13, 尾 -cateninine GSK-3 尾 protein in chondrocytes of moderate exercise group was significantly lower than that in model control group and Bax protein expression was significantly higher than that in model control group. The expression of Bcl-2MMP _ 2 MMP-13, 尾 -cateninine GSK-3 尾 protein in chondrocytes in moderate exercise group was significantly lower than that in low-intensity exercise group (P0.01). By regulating the expression of Bcl-2 and Bax protein, aerobic intensity decreased the apoptosis of chondrocytes in arthritis, and down-regulated MMP-2 / MMP-13 and decreased the damage of chondrocytes. The mechanism was related to the down-regulation of Wnt- 尾 -catenin signaling pathway.
【作者单位】：郑州大学体育学院;
【基金】：郑州大学体育学院体育教育省级特色专业资助(教高[2012]979号)
【分类号】：R684.3

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