浙江省三个斑鳜野生群体线粒体DNA细胞色素b基因序列的遗传变异

发布时间：2018-06-25 17:51

本文选题：斑鳜 + 细胞色素b基因　；参考：《浙江农业学报》2017年10期

【摘要】：对钱塘江上游江山、乌溪江段和瓯江上游云和段3个斑鳜(Siniperca scherzeri Steindachner)群体线粒体DNA细胞色素b(Cyt b)基因片段进行了扩增和测序,比较并分析了其遗传多样性和群体遗传分化。结果显示,在斑鳜Cytb基因965 bp的部分序列中,A+T含量(52.11%)略高于G+C含量(47.89%),77个个体中发现了16个多态性位点,8种单倍型。江山群体、乌溪江群体和云和群体的单倍型多样性分别为0.570±0.078、0.668±0.067、0.667±0.032,核苷酸多样性分别为0.001 8±0.000 3、0.001 9±0.000 2、0.004 3±0.000 6。江山群体与乌溪江群体间的Fst为-0.005 16(P0.05),表明同为钱塘江上游的两群体没有明显分化;但云和群体与江山群体、乌溪江群体间Fst分别为0.191 23(P0.01)和0.178 10(P0.01),表明瓯江上游与钱塘江上游斑鳜群体存在显著的分化。
[Abstract]:The mitochondrial cytochrome b (Cyt b) gene fragments of Siniperca scherzeri Steindachner in Jiangshan, Wuxi and Oujiang upper reaches of Qiantang River were amplified and sequenced. The genetic diversity and population differentiation of Siniperca scherzeri Steindachner were compared and analyzed. The results showed that the A T content (52.11%) in the 965 BP sequence of Cytb gene was slightly higher than that in G C (47.89%). Among 77 individuals, 8 haplotypes were found in 16 polymorphic loci. The haplotype diversity and nucleotide diversity of Jiangshan population, Wuxijiang population and Yunhe population were 0.570 卤0.078 卤0.067 卤0.032 and 0.001 8 卤0.000 3,0.001 9 卤0.000 2 卤0.004 3 卤0.000 6, respectively. The Fst between Jiangshan population and Wuxi River population was -0.005 16 (P0.05), which indicated that there was no obvious differentiation between the two groups, but the Yunhe population and Jiangshan population did not differentiate obviously. The FST of the populations in Wuxi River was 0.191 (P0.01) and 0.178 10 (P0.01) respectively, which indicated that there was significant differentiation between the upper reaches of Oujiang River and the upper reaches of Qiantang River in Siniperca chuatsi (Siniperca chuatsi) populations.
【作者单位】：浙江省水产技术推广总站;
【基金】：2013年度浙江省农业标准化研究项目“浙江省斑鳜种质特性研究”
【分类号】：S917.4

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