不同剂量川芎-当归、三棱-莪术对AS小鼠Cyr61、Sgk1、Ch25h基因表达水平的影响

发布时间：2018-06-26 02:58

本文选题：动脉粥样硬化 + 活血化瘀药　；参考：《中国中医基础医学杂志》2017年07期

【摘要】：目的:探讨不同剂量川芎-当归、三棱-莪术对载脂蛋白E(Apoe)基因缺陷小鼠动脉粥样硬化(AS)模型主动脉组织Cyr61、Sgk1、Ch25h基因表达水平的影响。方法:将64只5周龄Apoe基因缺陷小鼠按随机数字表法分为模型对照组、阿托伐他汀钙组、低活血药组、高活血药组、低破血药组、高破血药组6组,造模成功后分别灌胃给药连续8周,处理动物并用基因芯片技术检测小鼠主动脉组织中差异表达基因,并通过RT-PCR法进行验证。结果:川芎-当归、三棱-莪术均能下调Cyr61、Sgk1、Ch25h基因表达水平且存在量效关系。结论:活血药(当归、川芎)、破血药(三棱、莪术)具有抗动脉粥样硬化的作用,其作用机制可能与调节主动脉Cyr61、Sgk1、Ch25h基因表达水平有关。
[Abstract]:Aim: to investigate the effects of different doses of Ligusticum chuanxiong, Angelica sinensis and Rhizoma Zedoary on the expression of Cyr61Sgk1 Ch25h gene in aorta of mice with deficiency of apolipoprotein E (Apoe) gene. Methods: 64 5-week-old Apoe gene deficient mice were randomly divided into 6 groups: model control group, Atto vastatin calcium group, low blood activating drug group, high blood activating drug group, low blood breaking drug group and high blood breaking drug group. After the successful establishment of the model, the mice were given intragastric administration for 8 weeks respectively. The differentially expressed genes in the aorta of mice were detected by gene chip technique, and the results were verified by RT-PCR. Results: both Rhizoma Chuanxiong and Angelica sinensis and Zedoary zedoary could down-regulate the expression of Cyr61 Sgk1 Ch25h gene and had dose-effect relationship. Conclusion: Radix angelica sinensis, Rhizoma Chuanxiong and Rhizoma Curcumae have anti-atherosclerosis effects, and the mechanism may be related to regulating the expression of Cyr61Sgk1Sgk1Ch25h gene in aorta.
【作者单位】：湖南中医药大学;中国中医科学院中医基础理论研究所;湖南中医药大学第一附属医院;
【基金】：国家自然科学基金面上项目(81273752)-活血、破血药对ApoE基因缺陷小鼠AS模型血管内皮细胞增殖与凋亡的差异研究
【分类号】：R285.5

【相似文献】