人食管鳞状细胞癌中微RNA-1250及其宿主基因AATK的表达及甲基化状态分析

发布时间：2018-06-28 11:10

本文选题：食管肿瘤 + DNA甲基化　；参考：《肿瘤》2017年05期

【摘要】：目的:探讨微RNA-1250(microRNA-1250,miR-1250)及其宿主基因细胞凋亡关联酪氨酸激酶(apoptosis-associated tyrosine kinase,AATK)在食管鳞状细胞癌(esophageal squamous cell carcinoma,ESCC)发生和发展中的作用。方法:分别采用实时荧光定量PCR法和甲基化特异性PCR法检测AATK基因在食管癌细胞系和ESCC组织及其相应癌旁正常组织中的表达情况和甲基化状态,同时采用实时荧光定量PCR法检测miR-1250在ESCC组织及其相应癌旁正常组织中的表达情况。然后,分析miR-1250表达和AATK基因表达及其甲基化状态分别与ESCC组织病理学特征的关系,以及三者之间的相关性。结果:5-杂氮-2’-脱氧胞苷(5-aza-2’-deoxycytidine,5-Aza-dC)处理后,4株食管癌细胞系中AATK基因表达水平明显升高(P值均0.05),并且其甲基化程度明显降低。在ESCC组织中,AATK mRNA平均表达量低于癌旁正常组织(P0.05),AATK基因甲基化率明显高于癌旁正常组织(P0.05);AATK mRNA相对表达量和AATK基因甲基化率均与淋巴结转移、组织分化程度及TNM分期密切相关(P值均0.05),而与患者年龄和性别无关(P值均0.05)。同时,ESCC组织中miR-1250相对表达量明显低于癌旁正常组织(P0.05),并与淋巴结转移、组织分化程度及TNM分期密切相关(P值均0.05),而与患者年龄和性别无关(P值均0.05)。ESCC组织中miR-1250与AATK mRNA表达明显相关(P0.05),而且miR-1250和AATK mRNA表达与AATK基因甲基化状态也均明显相关(P值均0.05)。结论:ESCC的发生和发展可能与miR-1250和AATK低表达以及AATK基因高甲基化状态有关,而且miR-1250与AATK mRNA表达具有一致性。
[Abstract]:Aim: to investigate the role of microRNA-1250 (microRNA-1250 miR-1250) and its host gene apoptosis-associated tyrosine kinase (apoptosis-associated tyrosine kinase- AATK) in the pathogenesis and development of esophageal squamous cell carcinoma (esophageal squamous cell carcinomaESCC). Methods: the expression and methylation status of AATK gene in esophageal carcinoma cell line, ESCC and its adjacent normal tissues were detected by real-time fluorescence quantitative PCR and methylation specific PCR, respectively. The expression of miR-1250 in ESCC and its adjacent normal tissues was detected by real-time fluorescent quantitative PCR. Then, the relationship between the expression of miR-1250 and AATK gene and the histopathological characteristics of ESCC was analyzed. Results the expression level of AATK gene in 4 esophageal cancer cell lines treated with 5 aza-2aza-deoxycytidine 5-Aza-dC was significantly increased (P < 0.05), and its methylation degree was significantly decreased. The average expression of AATK mRNA in ESCC was significantly lower than that in adjacent normal tissues (P0.05), and the methylation rate of AATK mRNA and AATK gene methylation were significantly higher than those in adjacent normal tissues (P0.05) and lymph node metastasis. Tissue differentiation and TNM stage were closely related (P 0.05), but not related to age and sex (P 0.05). At the same time, the relative expression of miR-1250 in ESCC was significantly lower than that in adjacent normal tissues (P0.05), and the expression of miR-1250 was associated with lymph node metastasis. Tissue differentiation and TNM stage were closely correlated (P 0.05), but not related to age and sex (P 0.05). MiR-1250 was significantly correlated with AATK mRNA expression in ESCC (P0.05), and miR-1250 and AATK mRNA expression were significantly correlated with AATK gene methylation (P 0.05). Conclusion the occurrence and development of the cell carcinoma may be related to the low expression of miR-1250 and AATK and the hypermethylation of AATK gene, and miR-1250 is consistent with the expression of AATK mRNA.
【作者单位】：河北医科大学第四医院河北省肿瘤研究所;
【基金】：国家自然科学基金资助项目(编号:81472335) 河北省自然科学基金资助项目(编号:H2015206196)~~
【分类号】：R735.1

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