BBC3基因表达与肺鳞状细胞癌预后的相关性及其分子机制研究

发布时间：2018-06-28 11:56

本文选题：BBC + 肺　；参考：《重庆医学》2017年19期

【摘要】：目的验证BCL2绑定组件3(BBC3)基因表达与肺鳞状细胞癌(LUSC)术后生存时间的相关性,探究其分子机制。方法利用实时荧光定量PCR(qRT-PCR)技术检测BBC3基因在39例LUSC患者肿瘤组织标本内的表达,并对患者进行随访,收集临床生存资料。使用Kaplan-Meier法结合log rank检验进行生存分析,使用COX比例风险模型进行多因素生存分析。在NCI-H226细胞系内过表达BBC3基因,利用噻唑蓝(MTT)法及流式细胞术检测其表达对细胞增殖及凋亡的影响。结果 BBC3的表达与肿瘤是否转移(r=0.556,P=0.023)、肿瘤大小(r=0.532,P=0.042)、T分期(r=0.551,P=0.021)及TNM分期(r=0.524,P=0.047)明显相关。Kaplan-Meier法结合log rank检验发现BBC3表达与患者生存时间明显相关,BBC3高表达者生存时间明显高于BBC3低表达者(χ~2=7.542,P=0.006)。COX比例风险模型分析发现,肿瘤是否转移、肿瘤T分期、TNM分期及BBC3表达均独立且明显影响患者的生存时间(P0.05)。重组BBC3质粒组凋亡细胞比例明显高于对照组与空载质粒组,差异均有统计学意义(P0.05)。结论 BBC3基因表达可抑制肿瘤细胞增殖、促进凋亡,与LUSC预后具有明显相关性,可能作为判别LUSC术后生存预测的的潜在辅助性分子标志物。
[Abstract]:Objective to investigate the relationship between BCL2 binding module 3 (BBC3) gene expression and survival time after operation of lung squamous cell carcinoma (LUSC). Methods Real-time quantitative PCR (qRT-PCR) was used to detect the expression of BBC3 gene in tumor tissues of 39 patients with LUSC. The patients were followed up and clinical survival data were collected. Kaplan-Meier method combined with log rank test was used for survival analysis and Cox proportional risk model was used for multivariate survival analysis. BBC3 gene was overexpressed in NCI-H226 cell line. MTT assay and flow cytometry were used to detect the effect of BBC3 gene expression on cell proliferation and apoptosis. Results the expression of BBC3 was significantly correlated with tumor metastasis (rn 0.556 P 0.023), tumor size (rn 0.532P 0.042) T stage (rn 0.551T 0.021) and TNM stage (rn 0.524 PnP 0.047). Kaplan-Meier method combined with log rank test showed that BBC3 expression was significantly correlated with survival time of patients with high expression of BBC3 than that of patients with high expression of BBC3.Methods Kaplan-Meier method combined with log rank test showed that the survival time of patients with high expression of BBC3 was significantly higher than that of patients with high expression of BBC3. Patients with low expression (蠂 2 + 7.542 P < 0.006). Cox proportional risk model analysis showed that, Tumor metastasis, tumor T stage, TNM stage and BBC3 expression were independent and significantly affected the survival time of patients (P0.05). The percentage of apoptotic cells in recombinant BBC3 plasmid group was significantly higher than that in control group and no-load plasmid group (P0.05). Conclusion BBC3 gene expression can inhibit the proliferation of tumor cells and promote apoptosis, which is closely related to the prognosis of LUSC, and may be a potential auxiliary molecular marker for predicting survival after LUSC.
【作者单位】：重庆医科大学附属永川医院胸心外科;重庆医科大学附属永川医院肾病风湿科;重庆市荣昌区人民医院内科;
【基金】：重庆市科委一般项目(cstc2016jcyjA0343) 重庆市卫生局科研面上项目(2015MSXM058) 重庆市永川区自然科学基金(Ycstc,2015nc5004) 重庆医科大学附属永川医院院内课题(YJZQN201529,YJSCI201504)
【分类号】：R734.2

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