导入iaaM与ipt双价基因的转基因大豆检测及表达效果研究

发布时间：2018-06-28 14:51

  本文选题：转基因 + 色氨酸单加氧酶　； 参考：《辽宁工业大学学报(自然科学版)》2016年06期

【摘要】：大豆是我国的重要的油料作物和经济作物,但其单产却远远低于欧美国家,因此提高其单产育种对国民的生产实践具有重要的意义。主要通过GUS组织化学染色分析、PCR检测、Southern blot检测技术对转iaa M与ipt双价基因的T1代转基因大豆进行检测,结果表明有5个株系iaa M和ipt基因以单拷贝的形式整合进了大豆基因组。采用半定量RT-PCR检测表明有4个株系外源双价基因高效表达。最后经产量性状考种结果表明:有3个株系的表现较好,其中株系4增产12.85%。
[Abstract]:Soybean is an important oil crop and cash crop in China, but its yield per unit is much lower than that in Europe and America. Gus histochemical staining was used to detect T 1 transgenic soybean with iaa M and ipt bivalent genes. The results showed that 5 lines iaa M and ipt genes were integrated into soybean genome in single copy form. Semi-quantitative RT-PCR analysis showed that there were 4 lines with high expression of exogenous bivalent genes. At last, the results of yield test showed that three lines had better performance, among them, line 4 increased the yield by 12.85kum.
【作者单位】： 朝阳师范高等专科学校;
【基金】：辽宁省教育厅课题资助(L2013431)
【分类号】：S565.1
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本文编号：2078342