小桐子糖原合成激酶基因JcGSK的克隆与表达分析

发布时间：2018-06-29 23:15

本文选题：小桐子 + JcGSK　；参考：《西北植物学报》2017年02期

【摘要】：以小桐子(Jatropha curcas L.)cDNA为模版,克隆了JcGSK基因的CDS序列。序列分析表明,JcGSK基因包含1 230bp完全阅读框(ORF),编码409个氨基酸。预测其编码蛋白质的相对分子量为46.33kD,理论等电点为8.58。Blast搜索结果及进化分析结果表明,JcGSK蛋白与巴西橡胶树GSK蛋白的氨基酸序列一致性最高(94%)且亲缘关系最近;JcGSK基因编码的蛋白具有一个蛋白激酶特有的结构域。组织表达结果显示,JcGSK基因在小桐子根、茎、叶、花、果皮和种子中都有表达,且在根中表达量最高。小桐子幼苗在NaCl、ABA、PEG、低温和机械损伤处理后JcGSK基因表达量有不同程度的上调,推测其参与小桐子非生物胁迫响应和信号传导过程。JcGSK基因在种子中也有较高表达,在种子发育过程中表达量的变化与种子生长发育趋势基本一致,推测JcGSK基因也参与调控小桐子种子的生长发育。
[Abstract]:Using Jatropha curcas L. cDNA as template, the CDS sequence of JCGSK gene was cloned. Sequence analysis showed that the JcGSK gene contained a complete reading frame (ORF) of 1 230bp, encoding 409 amino acids. The predicted relative molecular weight of the encoded protein is 46.33 KD, the theoretical isoelectric point is 8.58. Blast search results and evolution analysis results show that the amino acid sequence of JcGSK protein and GSK protein of Hevea brasiliensis is the highest (94%), and the closest genetic relationship is JcGSK gene. The encoded protein has a domain specific to protein kinase. The results of tissue expression showed that JcGSK gene was expressed in roots, stems, leaves, flowers, pericarp and seeds, and the highest expression was found in roots. The expression of JcGSK gene was up-regulated in different degree after the treatment of low temperature and mechanical injury. It was speculated that the JcGSK gene was also highly expressed in seeds in response to abiotic stress and signal transduction process. The change of expression level in seed development was basically consistent with the trend of seed growth and development. It was speculated that JcGSK gene was also involved in regulating the growth and development of jatropha seed.
【作者单位】：昆明理工大学现代农业工程学院;昆明理工大学建筑与城市规划学院;云南农业大学园林园艺学院;云南师范大学生命科学学院;
【基金】：国家自然科学基金(31460355)
【分类号】：S794.9;Q943.2

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