普安银鲫胚胎发育中脂质代谢酶基因的表达及葡萄糖的影响

发布时间：2018-06-30 04:21

本文选题：普安银鲫 + 胚胎发育　；参考：《水产科学》2017年03期

【摘要】：采用荧光定量PCR技术检测了普安银鲫胚胎发育中乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶基因表达的特点及葡萄糖浸泡的影响,以探讨乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶基因在普安银鲫胚胎发育中的表达及mRNA表达水平及葡萄糖对这4种基因mRNA表达量的影响。试验结果显示,乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶基因在普安银鲫的成熟卵期均有表达,且随胚胎发育而上升。在15g/L葡萄糖处理组中,乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶从原肠期至出膜前期mRNA表达量升高。在原肠期、晶体出现期和出膜前期,葡萄糖能显著上调脂肪酸合成酶和乙酰辅酶A羧化酶αmRNA的表达(P0.05)。在原肠期,葡萄糖能稍上调脂蛋白脂酶和肝脂酶mRNA的表达,但差异不显著(P0.05)。在晶体出现期和出膜前期,葡萄糖能显著上调脂蛋白脂酶和肝脂酶mRNA的表达(P0.05)。研究表明,乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶属母源性酶类,其合子基因在原肠期已经开始表达。适宜水平的葡萄糖促进脂肪酸合成酶和乙酰辅酶A羧化酶αmRNA的表达有利于脂肪酸合成酶和乙酰辅酶A羧化酶α的积累,为仔鱼出膜做准备;促进脂蛋白脂酶和肝脂酶mRNA表达有利于脂质的分解供能。
[Abstract]:The expression characteristics of acetyl coenzyme A carboxylase 伪, fatty acid synthase, lipoprotein lipase and liver lipase gene and the effect of glucose immersion on the expression of acetyl coA carboxylase 伪, fatty acid synthase, lipoprotein lipase and hepatic lipase were detected by fluorescence quantitative PCR. To investigate the expression of acetyl coA carboxylase 伪, fatty acid synthase, lipoprotein lipase and liver lipase gene in embryo development of crucian carp, and the effect of glucose on the mRNA expression of these four genes. The results showed that acetyl coenzyme A carboxylase 伪, fatty acid synthase, lipoprotein lipase and liver lipase genes were all expressed in the mature eggs of crucian carp, and increased with the development of embryo. In 15g / L glucose treated group, the mRNA expression of acetyl coA carboxylase 伪, fatty acid synthase, lipoprotein lipase and liver lipase increased from the prointestinal phase to the early stage of exfoliation. Glucose could significantly upregulate the expression of fatty acid synthase and acetyl coenzyme A carboxylase 伪 mRNA in the proto intestinal phase, lens appearance stage and early stage of membrane exudation (P0.05). Glucose could slightly upregulate the expression of lipoprotein lipase and hepatic lipase mRNA in proto intestinal phase, but the difference was not significant (P0.05). Glucose could significantly upregulate the expression of lipoprotein lipase and liver lipase mRNA at the stage of lens formation and pre-membrane exudation (P0.05). The results showed that acetyl coenzyme A carboxylase 伪, fatty acid synthase, lipoprotein lipase and liver lipase belong to maternal enzymes, and their zygote genes were already expressed in the proto intestinal phase. The appropriate level of glucose promoted the expression of fatty acid synthase and acetyl coA carboxylase 伪 mRNA, which was beneficial to the accumulation of fatty acid synthase and acetyl coenzyme A carboxylase 伪, and prepared for the release of larva membrane. Promoting the expression of lipoprotein lipase and liver lipase mRNA is beneficial to the decomposition and energy supply of lipids.
【作者单位】：贵州省瓮安县农村工作局;贵州大学动物科学学院水产科学系高原山地动物遗传育种与繁殖教育部重点实验室;
【基金】：国家自然科学基金资助项目(31160527)
【分类号】：S917.4

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