柴胡疏肝散对围绝经期综合征肝郁大鼠卵巢雌激素受体基因表达的影响

发布时间：2018-07-01 18:06

本文选题：柴胡疏肝散 + 围绝经期综合征　；参考：《世界中西医结合杂志》2016年07期

【摘要】：目的探讨柴胡疏肝散对围绝经期肝郁大鼠卵巢雌激素受体(estrogen receptor,ER)基因ERα和ERβ表达的影响,探讨柴胡疏肝散治疗围绝经期综合征的作用机制。方法 24只13月龄雌性自然衰老的围绝经期SD大鼠被随机分为围绝经期对照组(n=8)、围绝经期模型组(n=8)、柴胡疏肝散干预组(n=8),另取3月龄青年雌性大鼠做青年对照组(n=8)。利用慢性束缚法造围绝期综合征肝郁证模型,造模期间药物干预组给予柴胡疏肝散,其余各组大鼠给予生理盐水,连续灌胃共21d。采用实时荧光定量PCR法检测卵巢雌激素受体ERα和ERβ的表达。结果 ERα和ERβmRNA在各组大鼠的卵巢组织中均有表达,但ERα的表达略高于ERβ。与青年对照组比较,围绝经期对照组的ERα、ERβmRNA表达差异无统计学意义(P0.05)。与围绝经期对照组比较,围绝经期模型组的ERα、ERβmRNA表达显著下调(P0.05),而柴胡疏肝散干预组显著提高(P0.01)。结论柴胡疏肝散可能通过使雌激素受体基因表达上调,增强雌激素受体效应,而对围绝经期肝郁起改善作用,可能是其治疗围绝经期肝郁的作用机制之一。
[Abstract]:Objective to investigate the effect of chaihu Shugan powder on the expression of ER 伪 and ER 尾 in the ovary of rats with liver depression in peri-menopausal period, and to explore the mechanism of chaihu Shugan powder in the treatment of peri-menopausal syndrome. Methods Twenty-four postmenopausal SD rats aged 13 months were randomly divided into three groups: peri-menopausal control group (NN8), peri-menopausal model group (NC8), chaihu Shugan powder intervention group (NC8), and 3-month-old young female rats as control group (NN8). The model of liver depression was established by the method of chronic restraint. The drug intervention group was given Chaihu Shugan powder during the model making, and the other rats were given normal saline for 21 days. The expression of estrogen receptor ER 伪 and ER 尾 was detected by real time fluorescence quantitative PCR. Results ER 伪 and ER 尾 mRNA were expressed in ovarian tissues of all groups, but the expression of ER 伪 was slightly higher than that of ER 尾. Compared with the young control group, there was no significant difference in ER 伪 -ER 尾 mRNA expression in peri-menopausal control group (P0.05). Compared with the perimenopausal control group, the expression of ER 伪 and ER 尾 mRNA in the perimenopausal model group was significantly down-regulated (P0.05), while that in the chaihu Shugan powder intervention group was significantly increased (P0.01). Conclusion Chaihu Shugan Powder may enhance the estrogen receptor effect by up-regulating the expression of estrogen receptor gene, and improve the liver depression during the menopausal period, which may be one of the mechanisms of its treatment of peri-menopausal liver depression.
【作者单位】：福建中医药大学;
【基金】：国家自然科学基金项目(81302886,81173203) 福建省自然科学基金项目(2013J01336) 福建中医药大学校管课题资助项目(X701120018)
【分类号】：R285.5

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