巴西橡胶树PEPRK基因家族成员的鉴定和表达分析

发布时间：2018-07-01 19:14

本文选题：巴西橡胶树 + PEPRK　；参考：《热带作物学报》2017年01期

【摘要】：PEP羧化酶激酶相关激酶(PEP carboxylase kinase-related kinases,PEPRKs)是CDPK-Sn RK超家族的一员,目前对其生理功能尚不清楚。本研究以拟南芥和杨树的PEPRK序列作为探针,对橡胶树和其他5种植物(木薯、水稻、杨树、蓖麻和拟南芥)的基因组和转录组数据进行全面搜索,共鉴定得到25个PEPRK基因家族成员,其中包括5个橡胶树PEPRK基因,命名为Hb PEPRK1-5。序列分析发现橡胶树PEPRK和其他植物类似,在序列中部含有一个相对比较保守的激酶结构域,而两端的同源性相对较低。在基因结构和进化方面,PEPRK主要分为2个大的分支,相同分支的成员在结构域和内含子分布上更为相似,其中橡胶树和木薯PEPRK在进化上具有较近的亲缘关系。在基因的表达方面,Hb PEPRK1主要在稳定期的叶片中表达,Hb PEPRK2和Hb PEPRK5在树叶、树皮和胶乳中均有表达,Hb PEPRK4主要在种子中表达;另外,研究发现橡胶树PEPRK家族成员的表达还与叶片发育、乙烯利刺激、真菌侵染和低温胁迫具有一定的相关性。
[Abstract]:PEP carboxylase kinase-related kinase associated kinases (PEPRKs) are members of the CDPK-Sn RK superfamily. In this study, genomic and transcriptional data of five other plants (cassava, rice, poplar, castor and Arabidopsis thaliana) were searched using PEPRK sequences of Arabidopsis thaliana and poplar as probes. A total of 25 members of PEPRK gene family were identified, including 5 PEPRK genes in Hevea, named HB PEPRK1-5. Sequence analysis showed that PEPRK was similar to other plants and contained a relatively conserved kinase domain in the middle of the sequence, with relatively low homology at both ends. PEPRK is divided into two branches in gene structure and evolution, and the members of the same branch are more similar in the distribution of domain and intron, among them, there is a close phylogenetic relationship between Hevea and cassava PEPRK. In gene expression, Hb PEPRK2 and HB PEPRK5 were mainly expressed in leaves, bark and latex, and HbPEPRK4 was mainly expressed in seeds. In addition, it was found that the expression of HbPEPRK2 and HB PEPRK5 was also related to leaf development. Ethephon stimulation, fungal infection and low temperature stress were correlated.
【作者单位】：中国热带农业科学院橡胶研究所;海南大学农学院;
【基金】：国家自然科学基金项目(No.31400565)
【分类号】：S794.1

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