三唑磷诱导的酰基辅酶A氧化酶基因对褐飞虱生殖的影响

发布时间：2018-07-03 00:50

本文选题：褐飞虱 + 脂代谢　；参考：《扬州大学》2016年硕士论文

【摘要】：褐飞虱Nilaparvata lugens (Stal)是亚洲地区一种远距离迁飞性的水稻害虫。褐飞虱再猖獗涉及到毒物兴奋效应及补偿作用、农药刺激害虫生殖的生理机制和药剂刺激害虫生殖的分子机制等。本文尝试通过对褐飞虱脂肪体蛋白质分析和RNA干扰(RNA interference, RNAi)方法研究三唑磷对褐飞虱脂代谢及生殖的影响。1.两种药剂处理的褐飞虱脂肪体脂质代谢分析在昆虫的生长发育、生殖过程中,脂质代谢起着重要的作用,脂肪体是褐飞虱的代谢中心。本实验使用蛋白质组学分析方法,研究了三唑磷和茚虫威处理后褐飞虱脂肪体中脂质代谢的变化。共检测出91个脂质代谢相关的差异蛋白,占注释到具体通路中差异蛋白的18.1%,其中重要的有胰脂肪酶甘油三酯、谷氨酰胺合成酶、乙酰胆碱酯酶、过氧化物酶酰基辅酶A氧化酶等。共19条显著差异的脂质代谢通路,占差异通路的8.5%,包括脂肪的消化和吸收、脂肪酸代谢等其他脂类代谢途径。重要的有不饱和脂肪酸的生物合成、甘油脂代谢、甘油脂代谢、脂肪酸延长、脂肪的消化和吸收、甘油磷脂代谢、类固醇激素的生物合成、磷酸肌醇代谢等。由此可见,三唑磷处理的褐飞虱脂肪体中,脂类的代谢有着显著的变化。2.干扰酰基辅酶A氧化酶基因对褐飞虱生殖的影响本试验分析研究了各种处理的褐飞虱的差异蛋白,结果显示三唑磷显著刺激了脂类代谢的酰基辅酶A氧化酶(Acyl-coenzyme A Oxidase, ACO)表达。在随后的RNAi实验中发现,取食含有dsACO人工饲料的褐飞虱的生殖力显著下降。TZP+dsACO处理与TZP和TZP+dsGFP相比,产卵量分别下降了30%和32%；产卵历期分别下降了24%和21%；雌成虫寿命分别下降了20%和26%。褐飞虱雌成虫体内粗脂肪也显著减少,TZP+dsACO处理与TZP相比,粗脂肪含量显著下降42%(从5.3mg到3.1mg)。褐飞虱体内各种脂肪酸含量也有不同程度的变化,TZP+dsACO处理组褐飞虱雌虫脂肪体中油酸、棕榈酸、亚油酸、硬脂酸、肉豆蔻酸、油酸、棕榈酸、肉豆蔻酸含量与对照相比均显著下降。除此之外,TZP+dsACO处理与TZP相比,蛋白质含量显著下降11.8%(从197ug/五头雌虫到174ug/五头雌虫)。表明ACO是TZP诱导刺激BPH生殖的基因之一。
[Abstract]:The Nilaparvata lugens (Stal) of brown planthopper (Stal) is a long-range migratory pest of rice in Asia. The re rampant of brown planthopper involves the effect of toxicant excitatory and compensation, the physiological mechanism of pesticide stimulation to the reproduction of pests and the molecular mechanism of the insecticide to stimulate the reproduction of the pest. This article attempts to analyze the protein of the fat body of the brown planthopper and the RNA dry. RNA interference (RNAi) method study the effect of three zazophos on lipid metabolism and reproduction of brown planthopper. The lipid metabolism analysis of the fat body of brown planthopper, treated by.1. two agents, plays an important role in the growth and development of the insects. The lipid body is the metabolic center of the brown planthopper. The changes in lipid metabolism in the fat bodies of three zazophos and ninophos were studied. 91 differential proteins related to lipid metabolism were detected, accounting for 18.1% of the differential proteins in the specific pathway, including triglyceride, glutamine synthase, acetylcholinesterase and peroxidase A. A total of 19 significant differences in lipid metabolic pathways, accounting for 8.5% of the differential pathways, including fat digestion and absorption, fatty acid metabolism, and other lipid metabolism pathways. The biosynthesis of unsaturated fatty acids, triglyceride metabolism, triglyceride metabolism, fatty acid elongation, fat digestion and absorption, glycerol phospholipid metabolism, steroid The biosynthesis of hormones and the metabolism of inositol phosphate. This shows that there is a significant change in the metabolism of lipids in the fat body of three zolophos treated brown planthopper,.2. interferes with the effect of the acyl coenzyme A oxidase gene on the reproduction of the brown planthopper. The expression of acyl coenzyme A oxidase (Acyl-coenzyme A Oxidase, ACO) in lipid metabolism. In the subsequent RNAi experiment, the reproductive capacity of the brown planthopper containing dsACO artificial diet decreased significantly and.TZP+dsACO treatment decreased by 30% and 32%, respectively, compared with TZP and TZP+dsGFP; the spawning calendar period decreased by 24% and 21%, respectively. The total fat decreased by 20% and 26%. of the female adults of the brown planthopper, and the content of crude fat decreased by 42% (from 5.3mg to 3.1mg) compared with TZP. The content of fatty acids in the body of brown planthopper also changed in varying degrees. The fatty acid, palmitic acid, linoleic acid and stearic acid in the fatty body of the brown planthopper of the TZP+dsACO treated group of brown planthopper. The content of myristic acid, oleic acid, palmitic acid and myristic acid decreased significantly with the ratio of photographing. In addition, the protein content decreased by 11.8% significantly compared with TZP (from 197ug/ five females to five 174ug/ females), indicating that ACO was one of the genes induced by TZP to stimulate BPH reproduction.
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S435.112.3

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