兴国红鲤AR基因的鉴定及MT暴露对幼鱼肝中AR和vtg表达的影响

发布时间：2018-07-05 13:31

本文选题：雄激素受体 + 兴国红鲤(Cyprinus　；参考：《淡水渔业》2016年06期

【摘要】：实验克隆了兴国红鲤(Cyprinus carpio var singuonensis)雄激素受体(androgen receptor,AR)基因的全长c DNA序列和卵黄蛋白原(vitellogenin,vtg)基因的部分c DNA序列,并对雌性个体组织及甲基睾丸酮(Methyltestosterone,MT)暴露下幼鱼肝胰腺(以下简称:肝)中AR和vtg的表达进行检测。兴国红鲤AR基因c DNA全长3 229 bp,包括104 bp的5'非编码区(untranslation region,UTR)、编码846个氨基酸的2 541 bp开放阅读框(open reading frame,ORF)和485 bp 3'UTR。氨基酸序列同源性分析表明,兴国红鲤AR与其他鲤科鱼类AR的同源性较高。组织表达特征研究表明,AR在雌性个体的肝、卵巢、中肾、肌肉和端脑中有表达,其中肝中的表达量最高;vtg主要在肝、端脑和鳃中表达。兴国红鲤幼鱼在50μg/L的MT中暴露4周后,采用qRT-PCR检测了肝中AR和vtg的表达情况。在处理第1、2周,AR的表达受到一定的抑制,而在第3、4周其表达量升高,但其表达无显著性变化;而vtg的表达量在第3、4周均有极显著的升高。
[Abstract]:The full-length c DNA sequence of the androgen receptor AR gene and the partial c DNA sequence of the vitellogenin VTG gene of Cyprinus carpio var singuonensis) were cloned. The expression of AR and vtg in the hepatopancreas of juvenile fish (hereinafter referred to as liver) was detected in female individual tissues and methyltestosterone (MT). The total length of AR gene of Xingguo Red Carp is 3 229 BP, including 104bp untranslation region UTR, 2 541bp open reading frame (open reading frame ORF) encoding 846 amino acids, and 485 BP 3G UTR. The amino acid sequence homology analysis showed that the AR of Xingguo red carp had high homology with that of other Cyprinidae fishes. The study of tissue expression showed that the expression of AR was found in the liver, ovary, mesonephrosis, muscle and telencephalon of female individuals, and the highest expression of VTG was found in liver, telencephalon and Gill. After exposed to 50 渭 g / L MT for 4 weeks, the expression of AR and vtg in liver was detected by qRT-PCR. The expression of AR was inhibited at the 1st week, but increased at the 3rd week, but the expression of vtg increased significantly at the 3th week, while the expression of vtg increased significantly at the 4th week.
【作者单位】：农业部淡水生物多样性保护重点实验室中国水产科学研究院长江水产研究所;上海海洋大学水产与生命学院;
【基金】：国家高技术研究发展计划项目(2011AA100401)
【分类号】：S917.4

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