金堂黑山羊FSH信号调节对下丘脑基因表达谱的影响

发布时间：2018-07-05 15:28

本文选题：金堂黑山羊 + FSH信号　；参考：《西南农业学报》2017年12期

【摘要】：【目的】本研究旨在搞清楚金堂黑山羊FSH信号调节机制的作用模式。【方法】将10只生殖周期相同的健康空怀的金堂黑山羊成年母羊随机分成2组,一组为对照组,一组为试验组。在发情后的第2天对试验组金堂黑山羊进行FSH肌肉注射100IU,并在注射24 h后,摘取金堂黑山羊的下丘脑;对照组发情后的第3天摘取下丘脑,分别提取他们的总RNA,进行表达谱测序。【结果】经测序分析:对照组和试验组分别得到有效序列26004442和25041098条;经统计分析后,得到归一化序列25338808和24413890条,与山羊基因组的符合率分别达85.31%和85.32%。比对后发现152个基因出现显著性差异表达,其中117个基因上调了表达,35个基因下调了表达。GO功能分类发现1个生物学过程出现了显著性差异。KEGG功能分类没发现调节途径发生了显著性改变。结果显示FSH信号通过负反馈调节的方式对下丘脑进行调节,除了少量的基因表达随着FSH信号强度的变化外,其主要的生物学过程,调节途径均无显著性变化,这说明FSH信号的负向调节要轻微很多。【结论】FSH信号通过负反馈调节的方式对下丘脑进行调节,相比正向调节,FSH信号的负向调节要轻微很多。
[Abstract]:[objective] to study the mechanism of FSH signal regulation in Jintang Black Goat. [methods] Ten healthy, empty pregnant adult ewe of Jintang Black Goat with the same reproductive cycle were randomly divided into two groups, one group was the control group. One group was an experimental group. On the second day after estrus, 100 IUs were injected intramuscularly into Jintang black goats in the experimental group, and hypothalamus of Jintang black goat was removed 24 hours after injection, and hypothalamus was removed on the 3rd day after estrus in the control group. Their total RNAs were extracted and their expression profiles were sequenced. [results] after sequencing, 26004442 and 25041098 effective sequences were obtained in the control group and 25041098 in the experimental group, and 25338808 and 24413890 normalized sequences were obtained after statistical analysis. The coincidence rates with goat genome were 85.31% and 85.32%, respectively. After comparison, 152 genes were found to be differentially expressed. Among them, 117 genes were up-regulated, 35 genes down-regulated .go functional classification showed a significant difference in biological process. KEGG functional classification did not find significant changes in regulatory pathways. The results showed that the hypothalamus was regulated by FSH signal through negative feedback regulation. Except for a few gene expression changes with the change of FSH signal intensity, there was no significant change in the main biological process and regulation pathway of FSH signal. [conclusion] the negative regulation of FSH signal in hypothalamus is much less than that of positive regulation in hypothalamus. [conclusion] the negative regulation of FSH signal is much less than that of positive regulation.
【作者单位】：四川省畜牧科学研究院;动物遗传育种四川省重点实验室;
【基金】：四川省应用基础研究项目“金堂黑山羊FSH信号通路调节机制的研究”(2015JY0008)
【分类号】：S827

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